| 陆雪,赵骅,王进,李爽,田雪蕾,王福如,田梅,余宁乐,刘青杰.南京192Ir放射事故患者照后第4年细胞遗传学随访[J].中华放射医学与防护杂志,2020,40(3):183-188 |
| 南京192Ir放射事故患者照后第4年细胞遗传学随访 |
| Cytogenetic analysis of the victim followed-up at 4 year after 192Ir radiation accident in Nanjing |
| 投稿时间：2019-06-05 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.03.005 |
| 中文关键词: 染色体畸变 荧光原位杂交 G显带 生物剂量估算 修正系数 |
| 英文关键词:Chromosome aberration Fluorescence in situ hybrdization G banding Biological dose estimation Correction coefficient |
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| 中文摘要: |
| 目的 用5种细胞遗传学方法对南京192Ir源放射事故患者进行照后第4年随访，筛选回顾性剂量估算指标，为探明电离辐射远后效应提供依据。方法 采集事故患者外周血，进行非稳定性染色体畸变（双着丝粒+着丝粒环）、微核和核质桥分析，应用荧光原位杂交技术及G显带方法检测染色体易位，并估算生物剂量。结果 荧光原位杂交技术及G显带方法检测染色体易位估算的剂量分别为1.45和1.21 Gy，与事故后短期内估算的生物剂量相近；非稳定性染色体畸变、微核、核质桥估算的剂量分别为0.56、0.45、0.41 Gy，低于事故后短期内估算的生物剂量，其修正系数与时间的变化规律符合幂函数模型。结论 荧光原位杂交技术及G显带方法检测染色体易位估算方法，适用于回顾性剂量估算，应用非稳定性染色体畸变进行回顾性剂量估算需用修正系数进行校正。 |
| 英文摘要: |
| Objective To screen the indicators of retrospective dose estimation, based on 5 cytogenetic methods to assess the victim followed-up at 4 year after 192Ir radiation accident in Nanjing. Methods The chromosome aberration (dic + r) assay, cytokinesis block micronucleus (MN) and nucleoplasmic bridge (NPB) assay, fluorescence in situ hybridization (FISH)-based and G banding-based translocation analysis were used to retrospective biological dose estimation. Results The estimated doses of FISH-based and G banding -based analysis were 1.45 and 1.21 Gy respectively, which was similar to the biological dose estimated short time after the accident. However, the estimated doses by chromosome aberration, micronucleus and nucleoplasmic bridge method were 0.56, 0.45 and 0.41 Gy respectively, which were lower than the corresponding biodose. Correction factors were used to adjust the biodose. Conclusions In the 4th years after exposure, the estimated biological doses by FISH-based and G banding-based translocation were consistent with the biodose.Therefore, the two methods were suitable for retrospective dose estimation, while correction factors should be considered in chromosome aberration method for retrospective dose estimation. |
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