| 戴宏,刘玉龙,冯骏超,卞华慧,陈炜博,王优优.双着丝粒染色体自动分析生物剂量估算研究[J].中华放射医学与防护杂志,2017,37(3):182-186 |
| 双着丝粒染色体自动分析生物剂量估算研究 |
| Study on the automatic analysis of dicentric chromosome biodosimetry |
| 投稿时间：2016-11-01 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.03.004 |
| 中文关键词: 电离辐射 双着丝粒染色体 自动分析 生物剂量估算 |
| 英文关键词:Ionizing radiation Dicentric chromosome Automatic scoring Biological dosimetry |
| 基金项目:江苏省卫生计生委2014-2015年度预防医学科研项目（Y2015024） |
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| 中文摘要: |
| 目的 基于遗传工作站，探讨建立双着丝粒染色体自动分析剂量-效应曲线，实现高通量自动化生物剂量估算。方法 使用60Co放射源对3名健康志愿者外周血进行离体照射，常规培养、制片。使用遗传工作站进行染色体中期分裂相采集及双着丝粒自动分析，并人工确认，拟合双着丝粒染色体自动分析剂量-效应曲线。使用另一组不同剂量照射的离体外周血样本对拟合的双着丝粒染色体自动分析剂量-效应曲线进行准确性验证。结果 拟合的双着丝粒染色体自动分析剂量-效应曲线为Y=0.018 06D2+0.012 79D+0.000 489 1（R2=0.961），该剂量-效应曲线可以准确地进行生物剂量估算，并且每例人工分析时间仅需数分钟。结论 成功地建立了新的双着丝粒染色体自动分析生物剂量估算方法，可以大量节省人工分析时间，对大规模核事故应急具有重要意义。 |
| 英文摘要: |
| Objective To investigate a dose response curve based on a genetic workstation with automatic analysis system of dicentric chromosome assay (DCA) for establishing a high speed dose estimation method.Methods Peripheral blood from three healthy volunteers was irradiated in vitro using 60Co γ-rays, and then lymphocytes were cultured and fixed on slides using the standard protocol for DCA. Dicentric chromosome in metaphase cells was analyzed automatically with the genetic workstation and confirmed manually, and the dose response curve of automated dicentric chromosome was fitted. Dicentric chromosome of another peripheral blood sample irradiated with different doses was manually analyzed to verify the accuracy of the above automated DCA. Results The yield of automated DCA was well fitted by an equation Y=0.018 06D2+0.012 79D+0.000 489 1 with a correlation coefficient R2=0.961. The biological dose of radiation could be accurately estimated by this dose response curve within a few minutes. Conclusions We had successfully established a new dosimetry method by analyzing dicentric chromesome automatically, which can save a lot of manual analysis time and hence has important significance for emergency rescue in nuclear accidents. |
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