| 张雨静,王琳,张嘉琪,等.小鼠腹部照射的FLASH效应及其与肠道菌群的关系[J].中华放射医学与防护杂志,2026,46(4):352-358.Zhang Yujing,Wang Lin,Zhang Jiaqi,et al.FLASH effect after abdominal X-ray irradiation in mice and its relationship with gut microbiota[J].Chin J Radiol Med Prot,2026,46(4):352-358 |
| 小鼠腹部照射的FLASH效应及其与肠道菌群的关系 |
| FLASH effect after abdominal X-ray irradiation in mice and its relationship with gut microbiota |
| 投稿时间：2025-10-10 |
| DOI：10.3760/cma.j.cn112271-20251010-00357 |
| 中文关键词: X射线|FLASH效应|肠道菌群 |
| 英文关键词:X-ray|FLASH effect|Gut microbiota |
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| 中文摘要: |
| 目的 探究X射线超高剂量率(FLASH)照射和常规(CONV)照射对小鼠胃肠道的影响及其与肠道菌群的关系。方法 将113只C57BL/6J小鼠按完全随机方法分为空白对照(Sham)组、FLASH(200 Gy/s)组和CONV(4 Gy/min)组,分别为9、52、52只,单次照射全腹,每组分别照射10、15、20、25 Gy(每个剂量分别为15、15、11、11只)。每天记录小鼠整体情况、体重变化以及死亡情况,分别在照射前以及照射后1、3、7、30 d收集小鼠新鲜粪便进行16S rDNA测序,评估肠道菌群的变化,分析FLASH照射与肠道菌群的关系。结果 接受20和25 Gy全腹照射的小鼠在照射后1周内全部死亡,而10 Gy照射小鼠均未死亡。15 Gy时,FLASH组的生存率为77.78%,而CONV组的生存率为55.56%(P ＞ 0.05)。CONV组15 Gy照射后第30天Shannon指数低于Sham组(q = 5.16,P ＜ 0.05),CONV组10 Gy照射后第7天Chao指数以及照射后第30天主坐标分析(PCoA)均与Sham组差异有统计学意义(q = 3.81,R = 0.45,P ＜ 0.05),但FLASH组的以上3个指标与Sham组差异均无统计学意义(P ＞ 0.05)。FLASH组10 Gy照射后的Chao指数和菌群失调指数较CONV组恢复更快(q = 3.81、4.06,P ＜ 0.05),并且FLASH组的菌群物种数变化幅度更小。Lactobacillus 和Akkermansia的丰度与照射方式以及照射后时间密切相关。结论 与CONV照射相比,FLASH照射可减少对肠道组织的损伤以及对肠道菌群的影响,Lactobacillus和Akkermansia等细菌可能与FLASH效应相关。 |
| 英文摘要: |
| Objective To investigate the effects of ultra-high dose rate FLASH irradiation and conventional (CONV) irradiation using X-rays on the gastrointestinal tract and the relationship between these effects and gut microbiota. Methods A total of 113 C57BL/6J mice were completely randomized into a control group, a FLASH irradiation group (200 Gy/s), and a conventional irradiation group (4 Gy/min), which were termed the Sham, FLASH, and CONV groups and comprising 9, 52, and 52 mice, respectively. The three groups, except for the Sham group, received a single whole-abdomen irradiation at doses of 10, 15, 20 and 25 Gy, which were applied to 15, 15, 11, and 11 mice, respectively. Daily monitoring was conducted to record the overall conditions, body weight changes, and mortality of the mice. Furthermore, fresh stool samples were collected pre-irradiation and at 1, 3, 7, and 30 d post-irradiation. Subsequently, 16S rDNA sequencing was conducted for these samples to assess changes in gut microbiota and analyze the relationship between FLASH irradiation and gut microbiota. Results The mice receiving 20 and 25 Gy whole-abdomen irradiation all died within one week post-irradiation, whereas those receiving 10 Gy irradiation all survived. Under a dose of 15 Gy, the survival rates of the FLASH and CONV groups were determined at 77.78% and 55.56%, respectively (P > 0.05). The CONV group displayed a lower Shannon index at 30 d after 15 Gy irradiation than the Shannon index of the Sham group (q = 5.16, P < 0.05). Furthermore, the Chao index at 7 d after 10 Gy of irradiation and the principal coordinate analysis (PCoA) result at 30 d after 10 Gy of irradiation of the CONV group showed statistically significant differences with the Chao index and PCoA result of the Sham group, respectively (q = 3.81, R = 0.45, P ＜ 0.05). In contrast, the three metrics of the FLASH group showed no significant differences with those of the Sham group (P > 0.05). Following 10 Gy irradiation, the FLASH group exhibited faster recovery rates of the Chao index and microbial dysbiosis index (MDI) (q = 3.81, 4.06, P ＜ 0.05), along with smaller fluctuations in the number of gut bacterial species, compared to the CONV group. Notably, the abundances of Lactobacillus and Akkermansia were intimately associated with both the irradiation method and post-irradiation time. Conclusions Compared to conventional irradiation, FLASH irradiation can induce reduced damage to intestinal tissue and produce less impact on gut microbiota. The abundances of bacteria such as Lactobacillus and Akkermansia may be associated with the FLASH effect. |
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