| 张娇,王勇懿,段敏,等.嗜酸性粒细胞缺失对急性放射性肺损伤的影响研究[J].中华放射医学与防护杂志,2026,46(4):344-351.Zhang Jiao,Wang Yongyi,Duan Min,et al.Impacts of eosinophil deficiency on acute radiation-induced lung injury[J].Chin J Radiol Med Prot,2026,46(4):344-351 |
| 嗜酸性粒细胞缺失对急性放射性肺损伤的影响研究 |
| Impacts of eosinophil deficiency on acute radiation-induced lung injury |
| 投稿时间：2025-01-21 |
| DOI：10.3760/cma.j.cn112271-20250121-00029 |
| 中文关键词: 嗜酸性粒细胞|γ射线|放射性肺损伤 |
| 英文关键词:Eosinophil|γ-ray|Radiation-induced lung injury |
| 基金项目: |
| 作者 | 单位 | E-mail | | 张娇 | 河北大学生命科学学院, 保定 071002
军事科学院军事医学研究院, 北京 100850 | | | 王勇懿 | 军事科学院军事医学研究院, 北京 100850 | | | 段敏 | 军事科学院军事医学研究院, 北京 100850 | | | 杨喆 | 军事科学院军事医学研究院, 北京 100850 | | | 黎文聪 | 军事科学院军事医学研究院, 北京 100850 | | | 郝延辉 | 军事科学院军事医学研究院, 北京 100850 | | | 左红艳 | 军事科学院军事医学研究院, 北京 100850 | | | 李杨 | 河北大学生命科学学院, 保定 071002
军事科学院军事医学研究院, 北京 100850 | leeyoung109@hotmail.com |
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| 中文摘要: |
| 目的 探讨嗜酸性粒细胞(EOS)缺失对急性放射性肺损伤(RILI)进程的影响。方法 通过敲除GATA-1启动子中高亲和力GATA结合位点,构建Δdbl.GATA基因编辑小鼠。将8~10周龄Δdbl.GATA雌性小鼠、Δdbl.GATA雄性小鼠、野生型(WT)C57BL/6J雌性和WT C57BL/6J雄性小鼠各29只分别按随机数表法分为对照组、照射后3d组和照射后7d组,全身照射,剂量为8.5 Gy。分别于照射后3和7 d统计小鼠肺指数,采用流式细胞术检测小鼠肺组织EOS占白细胞比值、小动物肺功能检测系统检测小鼠肺功能、HE染色观察小鼠肺组织病理变化、放射免疫法检测小鼠肺组织TNF-α、IL-5、IL-4的含量。结果 照射后WT C57BL/6J雌性和雄性小鼠肺组织EOS占白细胞比值均低于对照组,差异有统计学意义(t = 9.02~14.55,P < 0.001);Δdbl.GATA雌鼠肺组织EOS占白细胞比值显著低于WT C57BL/6J雌鼠(t = 4.66,P < 0.01)。照射后WT C57BL/6J雌性和雄性小鼠相对吸气峰值、相对呼气峰值、相对潮气量、相对每分通气量均显著低于照射前(t = 3.30~9.20,P < 0.05),Δdbl.GATA小鼠上述肺功能指标均高于WT C57BL/6J小鼠(t = -5.59~-2.61,P < 0.05)。照射后WT C57BL/6J小鼠肺组织TNF-α、IL-5、IL-4含量均高于对照组(t = -4.06~-2.68,P < 0.05),Δdbl.GATA雌鼠肺组织TNF-α、IL-5、IL-4的含量均低于WT C57BL/6J雌鼠(t = 2.12~3.23,P < 0.05)。两种小鼠肺脏指数、肺组织病理变化差异无统计学意义。结论 EOS缺失可减轻小鼠放射性肺损伤。 |
| 英文摘要: |
| Objective To explore the impacts of eosinophil (EOS) deficiency on the progression of acute radiation-induced lung injury (RILI) Methods The Δdbl.GATA gene-edited mice were constructed by knocking out the high-affinity GATA binding site within the GATA-1 promoter region. The 8 to 10-week-old female and male Δdbl.GATA, as well as female and male wild-type (WT) C57BL/6J mice, 29 each type, were grouped into the control, 3 d post-irradiation, and 7 d post-irradiation groups using the random number table method. Mice in the latter two groups were exposed to total body irradiation at 8.5 Gy. At 3 d and 7 d post-irradiation, the lung indices of mice were calculated, the EOS-to-leukocyte ratios in the lung tissue of the mice were detected using flow cytometry, and the pulmonary function of the mice was evaluated using a small animal lung function testing system. Furthermore, the histopathological changes in the lung tissue were observed through hematoxylin and eosin (H&E) staining, and the TNF-α, IL-5, and IL-4 levels in the lung tissue were quantified by radioimmunoassay. Results Post-irradiation, compared to the mice in the control group, both female and male WT C57BL/6J gene-edited mice showed significantly lower EOS-to-leukocyte ratios of the lung tissue, with statistically significant differences (t =9.02-14.55, P < 0.001). Compared to the female WT C57BL/6J mice, the female Δdbl.GATA gene-edited mice exhibited significantly lower EOS-to-leukocyte ratios in the lung tissue (t =4.66, P < 0.01). Post-irradiation, both female and male WT C57BL/6J mice showed significantly lower relative peak inspiratory flow, relative peak expiratory flow, relative tidal volume, and relative minute ventilation volume than those before irradiation (t =3.30-9.20, P < 0.05). The above lung function parameters of the Δdbl.GATA gene-edited mice were significantly higher than those of the WT C57BL/6J mice (t = -5.59 to -2.61, P < 0.05). Additionally, the TNF-α, IL-5, and IL-4 levels of the lung tissue of the WT C57BL/6J mice were significantly higher than those of the control group (t = -4.06 to -2.68, P < 0.05), while these parameters in the lung tissue of the female Δdbl.GATA gene-edited mice were significantly lower than those in the female WT C57BL/6J mice (t =2.12-3.23, P < 0.05). No significant differences were observed in the lung index and histopathological changes of lung tissue between Δdbl.GATA gene-edited and WT C57BL/6J mice. Conclusion EOS deficiency can alleviate radiation-induced lung injury in mice. |
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