| 王蜜蜜,田雪蕾,刘青杰.辐射响应lncRNAs的筛选及其作为组织损伤标志物在全身照射小鼠模型中的初步验证[J].中华放射医学与防护杂志,2026,46(3):238-245.Wang Mimi,Tian Xuelei,Liu Qingjie.Screening of radiation-responsive lncRNAs and their preliminary validation as tissue damage biomarkers in a whole-body irradiated mouse model[J].Chin J Radiol Med Prot,2026,46(3):238-245 |
| 辐射响应lncRNAs的筛选及其作为组织损伤标志物在全身照射小鼠模型中的初步验证 |
| Screening of radiation-responsive lncRNAs and their preliminary validation as tissue damage biomarkers in a whole-body irradiated mouse model |
| 投稿时间：2025-10-20 |
| DOI：10.3760/cma.j.cn112271-20251020-00366 |
| 中文关键词: LncRNAs 放射性组织损伤 电离辐射 生物标志物 |
| 英文关键词:LncRNAs Radiation-induced tissue injury Ionizing radiation Biomarker |
| 基金项目:国家自然科学基金(82173463) |
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| 摘要点击次数: 3981 |
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| 中文摘要: |
| 目的 探讨长链非编码RNA(lncRNAs)在全身照射小鼠模型中作为组织特异性或通用性辐射生物标志物的潜力。方法 利用国内外公共中英文数据库初步筛选人鼠同源且已知受辐射诱导的lncRNAs,利用60Co γ射线对小鼠进行0、2、4、6 Gy单次全身照射,于照射后48 h采集小鼠多个组织、血浆及血细胞样本,通过实时荧光定量PCR检测候选lncRNAs在不同组织、血浆及血细胞中的表达水平及其剂量依赖性变化。结果 通过文献检索共筛选出19个人鼠同源且已知受辐射诱导的lncRNAs,实时荧光定量PCR结果显示,Dino、Trp53cor1及Pvt1 在多个组织中受辐射诱导显著上调(t=3.69~10.11、2.56~11.74、2.22~12.98,P ＜ 0.05),其中Dino及Trp53cor1表现出良好的剂量依赖性(R2=0.92~0.99、0.82~0.99, P ＜ 0.05),且在血浆及血细胞中的变化趋势与组织水平相一致(t=2.46~5.54、5.02~7.14,R2=0.70~0.99、0.63~0.77, P ＜ 0.05);Pvt1在血浆中仅2 和4 Gy照射后表达上调,差异具有统计学意义(t=3.11、3.83, P ＜ 0.05),而在血细胞中趋势与之相反,呈现剂量依赖性下调(t=-13.77~-9.89, R2=0.86, P ＜ 0.05)。Tmevpg1仅在肺及大肠组织中受辐射诱导显著下调(t=-8.03~-2.61,P ＜ 0.05),并在肺组织中呈现良好的剂量依赖性改变(R2=0.76, P ＜ 0.05),表现出一定的组织特异性特征,且其在血浆及血细胞中的下调趋势与组织水平一致(Z = -2.88~ -2.42,t =-11.12~ -8.32,R2 = 0.81、0.74, P ＜ 0.05)。结论 Dino及Trp53cor1具备作为辐射暴露通用生物标志物的潜力;Tmevpg1具有作为肺和大肠辐射损伤早期微创预警标志物的潜力。 |
| 英文摘要: |
| Objective To explore the potential of long non-coding RNAs (lncRNAs) as tissue-specific or universal radiation biomarkers in a mouse model following whole-body irradiation. Methods A preliminary screening was conducted to identify lncRNAs that are homologous between humans and mice and known to be induced by radiation, utilizing public databases from both domestic and international sources. Mice were exposed to a single dose of whole-body irradiation using 60Co γ-rays at 0, 2, 4 and 6 Gy. At 48 h post-irradiation, multiple tissues, plasma and blood cells of irradiated mice were collected. The expression levels of candidate lncRNAs across these samples, along with their dose-dependent changes, were quantified by real-time quantitative PCR. Results A total of 19 lncRNAs that are homologous in both humans and mice and known to be radiation-induced were screened out through literature retrieval. Real-time quantitative PCR assay showed that Dino, Trp53cor1 and Pvt1 were significantly up-regulated in multiple tissues after irradiation (t = 3.69-10.11, 2.56-11.74, 2.22-12.98, P< 0.05). Notably, Dino and Trp53cor1 exhibited a clear dose-dependent response (R2 = 0.92-0.99, 0.82-0.99, P< 0.05), and they showed consistent up-regulation patterns in plasma and blood cells (t = 2.46-5.54, 5.02-7.14, R2 = 0.70-0.99, 0.63-0.78, P < 0.05). The expression of Pvt1 was significantly up-regulated at 2 Gy and 4 Gy in plasma (t = 3.11, 3.83, P < 0.05), whereas it exhibited a dose-dependent down-regulation in blood cells (t = -13.77 to -9.89, R2 = 0.86, P< 0.05). After irradiation, the expression of Tmevpg1 was significantly downregulated only in lung and colon (t = -8.03 to -2.61, P < 0.05), and exhibited a favorable dose-dependent change in lung tissue (R2 = 0.76, P < 0.05), indicating certain tissue-specific characteristics. Moreover, its down-regulation trend in plasma and blood cells was consistent with the tissue level (Z = -2.88 to -2.42, t = -11.12 to -8.32, R2 = 0.81, 0.74, P<0.05). Conclusions The changes in the expression levels of Dino and Trp53cor1 have the potential to serve as universal biomarkers for radiation exposure. The change in the expression level of Tmevpg1 suggests that it has the potential to serve as an early, minimally invasive warning marker for radiation damage to the lungs and large intestine. |
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