| 崔靓,陆星宇,商航,等.X射线FLASH胸部照射后小鼠远期认知行为与生存表现的纵向评估[J].中华放射医学与防护杂志,2026,46(2):183-191.Cui Liang,Lu Xingyu,Shang Hang,et al.A longitudinal evaluation of long-term cognitive behavior and survival performance of mice after FLASH X-ray chest irradiation[J].Chin J Radiol Med Prot,2026,46(2):183-191 |
| X射线FLASH胸部照射后小鼠远期认知行为与生存表现的纵向评估 |
| A longitudinal evaluation of long-term cognitive behavior and survival performance of mice after FLASH X-ray chest irradiation |
| 投稿时间：2025-07-07 |
| DOI：10.3760/cma.j.cn112271-20250707-00227 |
| 中文关键词: X射线 FLASH照射 辐射远端效应 行为学防护 远期效应 |
| 英文关键词:X-rays FLASH irradiation Radiation-induced abscopal effect (RIAE) Behavioral protection Long-term effect |
| 基金项目:国家自然科学基金(82272131) |
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| 中文摘要: |
| 目的 探讨kV级X射线FLASH照射对小鼠胸部照射后远期行为学表现的影响,并比较其与常规剂量率(CONV)照射在行为时序性特征上的差异。方法 选择5周龄雌性BALB/c小鼠,用随机数表法随机分为FLASH组(平均剂量率80 Gy/s)、CONV组(0.2 Gy/s)和假照射(SHAM)组,给予20 Gy胸部照射。照射后4、8和12个月,通过Y迷宫实验、旷场实验、新物体识别实验及筑巢实验评估其行为学表型。结果 生存分析显示,CONV组在照射后12个月生存率显著低于SHAM组(χ2 = 6.22, P < 0.05)。纵向行为学结果表明,FLASH组在旷场实验中的自发探索水平始终高于CONV组;照射后4个月,其中心探索时间显著高于CONV组(t = 4.29, P < 0.01),照射后12个月,其边缘停留时间显著低于CONV组(t = 4.86, P < 0.01)。Y迷宫实验中,FLASH组的空间记忆能力高于CONV组;照射后4个月,FLASH组的自发交替率显著高于CONV组(t = 3.31, P < 0.01),且CONV组的首次选择延迟时间显著长于FLASH组(t = 2.70, P < 0.05)。新物体识别实验显示,FLASH组能改善早期识别能力并实现长期功能保全,其行为学表现更接近于自然衰老过程;照射后4个月,FLASH组的识别指数与辨别指数值显著高于CONV组(t = 3.87、4.09, P < 0.01)。筑巢实验结果表明,照射后12个月,筑巢48 h FLASH组的筑巢评分显著高于CONV组(t = 5.01, P < 0.01),提示其筑巢能力保持更为完整。结论 12个月的观察期内,X射线FLASH照射在生存率、应激相关行为及运动探索等多个维度体现显著优势,有效缓解了胸部照射诱导的远期行为功能衰退。CONV照射虽在部分行为指标随月龄增长呈现动态变化,但仍伴随探索行为减少、生存率下降及本能行为受损等表现。 |
| 英文摘要: |
| Objective To investigate the effects of ultra-high dose rate (FLASH) chest irradiation with kilovoltage (kV) X-rays on the long-term behavioral performance of mice post-irradiation and to compare the temporal behavioral characteristics between the FLASH and conventional dose rate (CONV) irradiation. Methods Five-week-old female BALB/c mice were selected and, using the random number table method, they were randomly divided into three groups: a FLASH group (mean dose rate: 80 Gy/s), a CONV group (0.2 Gy/s), and a SHAM group. Mice in all groups received chest irradiation at a dose of 20 Gy. The behavioral assessment was performed at 4, 8, and 12 months post-irradiation using the Y-maze test, open field test, novel object recognition test, and nest building test. Results Survival analysis reveals a significantly lower 12-month post-irradiation survival rate in the CONV group than that in the SHAM group (χ2= 6.22, P<0.05). Longitudinal behavior assessment result indicate that in the open field test, mice in the FLASH group maintained higher spontaneous exploration levels than those in the CONV group. Specifically, at 4 months post-irradiation, mice in the FLASH group spent significantly more time exploring the center than those in the CONV group (t=4.29, P<0.01). In contrast, at 12 months post-irradiation, mice in the FLASH group spent significantly less time in the peripheral zones than those in the CONV group (t=4.86, P<0.01). The Y-maze test result indicate that mice in the FLASH group showed higher spatial memory ability than those in the CONV group, as evinced by significantly higher spontaneous alternation rates (t=3.31, P<0.01) and significantly shorter latency to first-arm entry (t=2.70, P<0.05) of the FLASH group compared to the CONV group at 4 months post-irradiation. The novel object recognition test demonstrates that mice in the FLASH group showed both early post-irradiation improvement in recognition ability and long-term functional preservation, with behavioral performance closer to the natural aging process. At 4 months post-irradiation, mice in the FLASH group displayed significantly higher recognition and discrimination indices than those of the CONV group (t=3.87, 4.09, P<0.01). The nest building test result indicate that mice in the FLASH group received significantly higher 48 h nest building scores than those in the CONV group at 12 months post-irradiation (t=5.01, P<0.01). This finding demonstrates that the FLASH group maintained a more intact nest building ability after irradiation. Conclusions During the 12-month observation period, FLASH X-ray irradiation offered significant advantages in multiple dimensions, including survival rate, stress-related behavior, and exploratory behavior, effectively alleviating the chest irradiation-induced functional decline in long-term behavior. In contrast, despite exhibiting age-dependent dynamic changes in some behavioral indicators, the CONV irradiation induced reductions in exploratory behavior and survival rate, as well as impaired instinctive behavior. |
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