| 崔智琳,杨镁楹,常晨旭,等.电磁场暴露对小鼠初级听觉皮层结构及功能的影响[J].中华放射医学与防护杂志,2026,46(1):5-12.Cui Zhilin,Yang Meiying,Chang Chenxu,et al.Effect of electromagnetic field exposure on the structure and function of the primary auditory cortex in mice[J].Chin J Radiol Med Prot,2026,46(1):5-12 |
| 电磁场暴露对小鼠初级听觉皮层结构及功能的影响 |
| Effect of electromagnetic field exposure on the structure and function of the primary auditory cortex in mice |
| 投稿时间：2024-11-21 |
| DOI：10.3760/cma.j.cn112271-20241121-00451 |
| 中文关键词: 电磁场 复合暴露 初级听觉皮层 听觉电生理 前脉冲抑制 |
| 英文关键词:Electromagnetic field Composite exposure Primary auditory cortex Auditory electrophysiological Prepulse inhibition |
| 基金项目: |
| 作者 | 单位 | E-mail | | 崔智琳 | 承德医学院基础医学院, 承德 067000
军事科学院军事医学研究院, 北京 100850 | | | 杨镁楹 | 军事科学院军事医学研究院, 北京 100850 | | | 常晨旭 | 军事科学院军事医学研究院, 北京 100850 | | | 石磊 | 军事科学院军事医学研究院, 北京 100850 | | | 王越 | 军事科学院军事医学研究院, 北京 100850 | | | 鲁纯雪 | 军事科学院军事医学研究院, 北京 100850 | | | 刘莹 | 军事科学院军事医学研究院, 北京 100850 | | | 郝延辉 | 军事科学院军事医学研究院, 北京 100850 | zuohy2005@126.com | | 李杨 | 军事科学院军事医学研究院, 北京 100850 | | | 左红艳 | 军事科学院军事医学研究院, 北京 100850 | |
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| 中文摘要: |
| 目的 探究静磁场和微波复合暴露对小鼠初级听觉皮层组织结构及功能的影响,为电磁场复合暴露生物学效应机制及防护研究提供新思路。方法 选取C57BL/6N小鼠,按照随机数表法分为对照组、静磁场暴露组、微波辐射组和电磁场复合暴露组,复合暴露组先经100 mT静磁场暴露1 h,再接受中心频率9.875 GHz、比吸收率(SAR)值2.58 W/kg的微波辐射15 min;静磁场暴露组仅行上述静磁场暴露,微波辐射组仅行上述微波辐射,对照组接受同等条件伪暴露。采用震惊条件反射前脉冲抑制(PPI)实验,研究电磁场复合暴露对听觉神经行为的影响。采用听性脑干反应(ABR)及畸变产物耳声发射实验(DPOAE),研究电磁场复合暴露对听觉电生理学功能的影响。采用光镜及透射电镜观察电磁场复合暴露对初级听觉皮层组织形态及超微结构的影响。结果 电磁场暴露后3、14 d,复合暴露组小鼠PPI(%)较暴露前和单一静磁场或微波暴露组均显著升高(P＜0.05);电磁场暴露后7、14、28 d,复合暴露组ABR I波潜伏期较对照组和和单一静磁场或微波暴露组均显著升高(P＜0.05),而DPOAE幅值显著降低(P＜0.05);ABR阈值未见明显变化;暴露后7 d,初级听觉皮层部分神经元变性凋亡、突触间隙模糊、胶质细胞线粒体肿胀,以复合暴露组最为明显。结论 静磁场和微波复合暴露可导致小鼠耳蜗外毛细胞功能受损、蜗神经上行传导通路功能受抑,初级听觉皮层组织病理学改变以及感觉运动门控功能异常,但对听力阈值无影响。 |
| 英文摘要: |
| Objective To explore the effect of combined exposure to static magnetic field (SMF) and microwave radiation on the structure and function of the primary auditory cortex (Au1) in mice, and to provide new insights into the mechanisms underlying biological effects and protection strategies for composite electromagnetic field (EMF) exposure. Methods C57BL/6N mice were randomly divided divided into four groups: control group, SMF exposure group, microwave exposure group, and a combined EMF expsoure group. The combined exposure group was exposed to a 100 mT SMF for 1 h, followed by microwave radiation with a center frequency of 9.875 GHz and SAR value of 2.58 W/kg for 15 min. The SME group received only the above SME exposure, the microwave group only received the above microwave radiation, and the control group underwent sham expsoure under identical conditions. The prepulse inhibition (PPI) of startle reflex test was used to evaluate auditory neurobehavioral changes induced by composite EMF exposure. Auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) were employed to acess alterlations in auditory electrophysiological function. Light microscopy and transmission electron microscopy (TEM) were utilized to observe morphological and ultrastructural changes in the PAC. Results At 3, 14 d post-exposure, the PPI percentage in the combined exposure group was significantly higher than that before exposure and in the single SMF or microwave exposure groups (P＜0.05). At 7, 14, and 28 d post-exposure, the latency of ABR wave I was significantly prolonged compared with the control group and the single exposure groups (P＜0.05), while the DPOAE amplitude was significantly reduced (P＜0.05), with no significant change in ABR threshold. Morphological and ultrastructural observations revealed neuronal degeneration and apoptosis, blurred synaptic clefts, and swelling of glial mitochondria in the Au1, with the most prominent changes detected in the combined exposure group at 7 d post-exposure. Conclusions Combined exposure to SMF and microwave radiation impairs the function of cochlear outer hair cells, inhibits the upward conduction function of the cochlear nerve pathway, induces histopathological changes in the Au1, and disrupts sensorimotor gating function, but exerts no effect on hearing threshold. |
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