| 王远旭,李金华,唐宇凤.X射线FLASH照射与常规剂量率照射对小鼠脑组织辐射损伤的影响[J].中华放射医学与防护杂志,2025,45(11):1069-1076.Wang Yuanxu,Li Jinhua,Tang Yufeng.Effects of X-ray FLASH irradiation and conventional-dose-rate irradiation on radiation-induced injury to the brain tissue of mice[J].Chin J Radiol Med Prot,2025,45(11):1069-1076 |
| X射线FLASH照射与常规剂量率照射对小鼠脑组织辐射损伤的影响 |
| Effects of X-ray FLASH irradiation and conventional-dose-rate irradiation on radiation-induced injury to the brain tissue of mice |
| 投稿时间：2025-07-10 |
| DOI：10.3760/cma.j.cn112271-20250710-00244 |
| 中文关键词: FLASH照射 超高剂量率 认知功能 |
| 英文关键词:FLASH irradiation Ultra-high dose rate Cognitive function |
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| 中文摘要: |
| 目的 基于国产X射线FLASH平台, 评估FLASH照射与常规照射对健康小鼠脑组织辐射损伤的影响。方法 将90只8周龄健康雄性C57BL/6J小鼠按随机数表法分为假照射(Sham)组、常规照射(CONV-RT)组及FLASH照射(FLASH-RT)组, 每组30只。采用单次20 Gy的全脑照射, FLASH照射剂量率为339 Gy/s, 常规照射为0.067 Gy/s。 通过水迷宫实验、旷场实验、尼氏染色及免疫组织化学分析胶质纤维酸性蛋白(GFAP)、离子钙结合衔接分子1(Iba-1), 评估不同放疗对神经功能与神经炎症的影响。结果 CONV组小鼠体质量与FLASH组小鼠在照后第8、9、10、11、12、24天差异具有统计学意义(q=2.82~5.31, P < 0.05)。照后4个月时FLASH组逃避潜伏期、平台穿越次数、中央区跨越次数、贴壁距离占比与CONV组之间差异具有统计学意义(q=3.72、2.92、3.28、2.97, P＜0.05)。尼氏染色定量结果提示, CONV组与FLASH组在海马CA3和DG区差异具有统计学意义(q=3.61、3.21, P＜0.05), 在CA1区差异无统计学意义(P＞0.05)。免疫组织化学结果显示, CONV组GFAP+阳性区域占比与FLASH组之间差异有统计学意义(q=2.28, P＜0.05), 但Iba-1+细胞数与FLASH组之间差异无统计学意义(P＞0.05)。结论 X-FLASH照射对脑组织造成的辐射损伤较常规剂量率轻, 具有良好的临床转化潜力。 |
| 英文摘要: |
| Objective To evaluate the effects of FLASH irradiation(FLASH-RT) and conventional irradiation (CONV-RT) on radiation-induced injury to the brain tissue of healthy mice using a domestically developed X-FLASH platform. Methods Ninety healthy 8-week-old male C57BL/6J mice were randomly divided into sham irradiation(Sham) group, CONV-RT group, and FLASH-RT group, with 30 mice in each group. A single dose of 20 Gy whole-brain radiotherapy was administered, with a dose rate of 339 Gy/s for FLASH radiotherapy and 0.067 Gy/s for conventional radiotherapy. The effects of different radiotherapy modalities on neurological function and neuroinflammation were assessed using water maze test, open field test, Nissl staining, and immunohistochemical analysis (GFAP, Iba-1). Results There were significant differences in body weight between the CONV group and the FLASH group at 8, 9, 10, 11, 12, and 24 d after radiotherapy (q=2.82-5.31, P<0.05). At 4 months after radiotherapy, there were significant differences between the FLASH group and the CONV group in escape latency, platform crossings, central zone crossings, and Thigmotaxis distance ratio (q=3.72, 2.92, 3.28, 2.97, P<0.05). Quantitative Nissl staining result revealed significant differences between the CONV group and the FLASH group in the CA3 and DG regions (q=3.61, 3.21, P<0.05), but not in the CA1 region (P>0.05). Immunohistochemical result showed a significant difference between the CONV group and the FLASH group in the percentage of GFAP+ positive area (q=2.28, P<0.05), but not in the number of Iba-1+ cells (P>0.05). Conclusions Compared to conventional radiotherapy, X-FLASH radiotherapy causes less radiation-induced injury to the brain tissue, thus holding a promising prospect for clinical translation. |
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