| 张天喜,叶海朋,俞慧娟,等.自动识别分析137 Cs γ射线照射诱导人外周血染色体畸变剂量-效应曲线的建立[J].中华放射医学与防护杂志,2025,45(9):858-862.Zhang Tianxi,Ye Haipeng,Yu Huijuan,et al.Establishing a dose-response curve for 137Cs γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis[J].Chin J Radiol Med Prot,2025,45(9):858-862 |
| 自动识别分析137 Cs γ射线照射诱导人外周血染色体畸变剂量-效应曲线的建立 |
| Establishing a dose-response curve for 137Cs γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis |
| 投稿时间：2025-05-14 |
| DOI：10.3760/cma.j.cn112271-20250514-00169 |
| 中文关键词: 自动识别分析 染色体畸变 剂量-效应曲线 γ射线 非稳定性染色体畸变分析 |
| 英文关键词:Automated analysis Chromosomal aberration Dose-response curve γ-rays Unstable chromosome aberration |
| 基金项目:杭州市医药卫生科技项目(A20230012);浙江省卫生信息学会科研计划项目(2024XHZN-Y02) |
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| 中文摘要: |
| 目的 自动识别分析137Cs γ射线诱导的人外周血淋巴细胞染色体畸变中,双着丝粒体(dic)+着丝粒环(r),并建立剂量-效应曲线。方法 采用剂量率为0.80 Gy/min的137Cs照射源对3名健康志愿者的外周血进行γ射线照射,照射剂量分别为0、0.5、0.75、1、1.5、2、3、4 和5 Gy。采用常规培养,自动收获仪收获、自动滴片仪制片,通过DCScore软件全自动分析dic+r, CABAS 软件建立"dic+r"剂量-效应曲线;对该曲线进行验证。结果 全自动分析拟合的剂量-效应曲线为y = 0.09 365+0.030 21D+0.025 31D2(R2 = 0.999 2)。用该曲线对照射的盲样进行剂量估算,与实际照射剂量偏差在20%以内。结论 采用自动识别分析系统成功构建137Cs γ射线诱导的"dic+r"剂量-效应曲线,在大规模辐射事故的核应急响应中具有很大的应用前景。 |
| 英文摘要: |
| Objective To establish a dose-response curve of dicentric chromosomes and centromeric rings (dic+r) in γ-ray irradiation-induced chromosomal aberrations in human peripheral blood lymphocytes through automated analysis. Methods Peripheral blood samples from three healthy donors were irradiated in vitro at doses of 0,0.5,0.75,1,1.5,2,3,4, and 5 Gy and a dose rate of 0.80 Gy/min using a 137Cs γ-ray source. Post-irradiation, lymphocytes were cultured based on standard protocols, harvested using an automatic cell harvester, and prepared on slides using an automatic slide preparation system. dic+r were analyzed fully automatically using the DCScore software, and a dose-response curve of dic+r was established through fitting and then validated using the CABAS software. Results The dose-response curve followed a linear-quadratic model, i.e., y = 0.093 65+0.030 21D+ 0.025 31D2 (R2 = 0.999 2), where y was the quantity of dic+r and D was the absorbed dose of γ-ray irradiation (Gy). Doses to samples for blind validation were estimated using this curve, yielding deviations of less than 24% from the actual irradiation doses. Conclusions The fully automated analysis of dic+r in 137Cs γ-ray irradiation-induced chromosomal aberrations, followed by the construction of the dose-response curve, holds significant potential for rapid, high-throughput biodosimetry in large-scale nuclear emergencies. |
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