| 李学达,田川,王文斐,等.3D放射性粒子模型在小鼠胶质瘤细胞中的应用[J].中华放射医学与防护杂志,2025,45(6):544-550.Li Xueda,Tian Chuan,Wang Wenfei,et al.Application of a 3D radioactive seed model to glioma cells in mice[J].Chin J Radiol Med Prot,2025,45(6):544-550 |
| 3D放射性粒子模型在小鼠胶质瘤细胞中的应用 |
| Application of a 3D radioactive seed model to glioma cells in mice |
| 投稿时间：2024-08-23 |
| DOI：10.3760/cma.j.cn112271-20240823-00317 |
| 中文关键词: 3D打印 模型 125I放射性粒子 |
| 英文关键词:3D printing Model 125I radioactive seed |
| 基金项目:国家重点研发计划中美政府间合作项目(2019DEY120100) |
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| 摘要点击次数: 5 |
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| 中文摘要: |
| 目的 应用放射治疗计划系统(TPS)及3D打印技术联合设计新型放射性粒子体外实验照射模型,并通过等剂量内外放疗小鼠胶质瘤细胞实验初步验证模型设计科学性。方法 应用TPS计划系统设计模型形状、计算粒子数量及位置,应用3D打印技术制作实验模型。选用GL261细胞系进行体外照射实验,分为对照组、外放疗(EBRT)组及内放疗(BT)组。其中EBRT组和BT组接受2、4和6 Gy不同剂量内外放疗照射后,检测细胞活力、细胞增殖、细胞内活性氧(reactive oxygen species, ROS)含量变化。结果 放射性粒子体外照射模型设计、制作成功,应用单光子发射计算机体层成像(SPECT)扫描验证模型内放射性分布均匀、无明显冷区。随着辐射剂量的增加,BT组和EBRT组的细胞活性均下降。与EBRT组相比,6 Gy剂量下BT组的细胞活性(51.33% vs. 22.00%,t = 10.94,P < 0.05)和克隆数(172.67 ± 13.11 vs. 53.67 ± 10.22, t = 8.73, P < 0.05)均显著降低,而ROS水平(102.52 ± 6.87 vs. 144.81 ± 6.01, t = -5.26, P < 0.05)明显升高。结论 基于TPS和3D打印技术的放射性粒子体外照射模型设计有效,为内外放疗机制研究提供了实验模型工具和研究方向。 |
| 英文摘要: |
| Objective To design a novel model for experiments on in vitro irradiation with radioactive seeds using a treatment planning system (TPS) and 3D printing technology and to preliminarily validate the design scientific rigor of the model via experiments on isodose brachytherapy (BT) and external beam radiotherapy (EBRT) on glioma cells in mice. Methods The TPS was employed to design the model's shape and calculate the number and positions of radioactive seeds, and 3D printing technology was utilized to fabricate the experimental model. The GL261 cell line was selected for in vitro irradiation experiments, with the mice divided into the control, EBRT, and BT groups. Mice in the EBRT and BT groups were treated with EBRT and BT, respectively, at doses of 2, 4, and 6 Gy. Then, changes in their cell viability, proliferation, and the level of intracellular reactive oxygen species (ROS) were assessed. Results The model for in vitro irradiation with radioactive seeds was successfully designed and fabricated. The single photon emission computed tomography (SPECT) verified a uniform radioactive distribution within the model, with no significant cold spots. The BT and EBRT groups displayed decreased cell viability with an increase in the radiation dose. Compared to the EBRT group, the BT group exhibited significantly reduced cell viability (51.33% vs. 22.00%, t= 10.94, P< 0.05) and clone counts (172.67 ± 13.11 vs. 53.67 ± 10.22, t = 8.73, P< 0.05), but a significantly increased level of ROS (102.52 ± 6.8 vs. 144.81 ± 6.01, t = -5.26, P< 0.05) at a dose of 6 Gy. Conclusions An effective model of in vitro irradiation with radioactive seeds is designed based on TPS and 3D printing technology. This provides an experimental model tool and target for research on the BT and EBRT mechanisms. |
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