| 李慧娴,陈慧峰,黄伟旭,等.阳江高本底辐射地区居民外周血差异表达mRNA的转录组学分析[J].中华放射医学与防护杂志,2025,45(5):392-398.Li Huixian,Chen Huifeng,Huang Weixu,et al.Transcriptomic analysis of differentially expressed mRNAs in the peripheral blood of residents in the Yangjiang high-background radiation area[J].Chin J Radiol Med Prot,2025,45(5):392-398 |
| 阳江高本底辐射地区居民外周血差异表达mRNA的转录组学分析 |
| Transcriptomic analysis of differentially expressed mRNAs in the peripheral blood of residents in the Yangjiang high-background radiation area |
| 投稿时间：2024-07-19 |
| DOI：10.3760/cma.j.cn112271-20240719-00269 |
| 中文关键词: 高本底辐射地区 低剂量电离辐射 转录组学 线粒体自噬 衰老 |
| 英文关键词:High background radiation area Low-dose ionizing radiation Transcriptomics Mitophagy Aging |
| 基金项目:广东省基础与应用基础研究基金(2019A1515011969,2022A1515012421,2023A1515010414,2025A1515012483);广州市科技计划项目(202002030031) |
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| 中文摘要: |
| 目的 探讨阳江高本底辐射地区(HBRA)与对照地区居民的差异表达基因、富集生物学过程及通路差异。方法 2023年7月至9月,采用二阶段随机抽样方法,分别抽取阳江HBRA居民(HBRA组)与恩平地区居民(对照组)各7名,根据环境γ外照射水平等计算外照射个人累积剂量;抽取调查对象外周血,采用高通量测序筛选差异表达基因进行基因本体学(GO)-生物学过程(BP)、京都基因与基因组大百科全书(KEGG)及基因富集分析(GSEA)检测。结果 HBRA组和对照组居民年龄比较差异无统计学意义(P =0.370);HBRA组和对照组外照射个人累积剂量分别为(99.59±20.07)和(33.82±10.61) mSv,差异具有统计学意义(t = -5.88,P =0.001);高通量测序结果显示,与对照组相比,HBRA组共有1 224个差异表达基因,其中包括32个衰老基因;GO-BP分析结果显示,HBRA组外周血差异表达基因生物学过程主要富集于细胞信号调节、细胞生物合成过程、细胞定位、细胞周期和细胞应激等;KEGG分析结果表明,HBRA组差异表达基因主要富集于趋化因子信号通路、MAPK信号通路、细胞周期、自噬和线粒体自噬等;GSEA分析结果显示,差异表达基因主要富集于细胞周期和线粒体相关基因。结论 HBRA居民外周血差异表达mRNA的相关生物学过程和通路与细胞周期、趋化因子信号通路、MAPK信号通路和线粒体自噬等有关。 |
| 英文摘要: |
| Objective To investigate differential gene expression, enriched biological processes, and pathway differences between residents of a high-background radiation area (HBRA), Yangjiang, and a control area—Enping. Methods Seven residents were selected from the HBRA (the HBRA group) and seven from the control area (the control group) using the two-stage random sampling method. The cumulative radiation dose for each individual was calculated based on the ambient gamma exposure levels. Peripheral blood samples were collected and analyzed via high-throughput sequencing to identify differentially expressed genes. Subsequent analyses included gene ontology (GO) for biological process (BP), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and gene set enrichment analysis (GSEA). Results The median ages of the HBRA and control groups had no statistically significant difference (P = 0.370). The cumulative external doses for the HBRA and control groups were (99.59±20.07) and (33.82±10.61) mSv, respectively. This difference was statistically significant (t = -5.88, P = 0.001). High-throughput sequencing identified 1 224 differentially expressed genes in the HBRA group, including 32 senescence-related genes, compared to the control group. The result of GO-BP analysis showed that these genes were predominantly enriched in cell signaling, biosynthesis, localization, cell cycle regulation, and cellular stress responses. KEGG analysis revealed significant enrichment in the chemokine and MAPK signaling pathways, as well as in pathways related to the cell cycle, autophagy, and mitophagy. Furthermore, GSEA analysis confirmed that the differentially expressed genes were mainly related to cell cycle regulation and mitochondrial functions. Conclusions Differentially expressed mRNAs are found in the peripheral blood of residents in the HBRA. These mRNAs are predominantly associated with key biological processes and pathways, including cell cycle regulation, chemokine and MAPK signaling pathways, and mitophagy. |
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