| 吴双,李志慧,洋雯茜,等.超高剂量率与常规剂量率脉冲电子线对秀丽隐杆线虫的影响[J].中华放射医学与防护杂志,2024,44(11):903-908.Wu Shuang,Li Zhihui,Yang Wenxi,et al.Effects of pulsed electron beams with ultra-high dose rate and conventional dose rate on Caenorhabditis elegans[J].Chin J Radiol Med Prot,2024,44(11):903-908 |
| 超高剂量率与常规剂量率脉冲电子线对秀丽隐杆线虫的影响 |
| Effects of pulsed electron beams with ultra-high dose rate and conventional dose rate on Caenorhabditis elegans |
| 投稿时间：2024-03-20 |
| DOI：10.3760/cma.j.cn112271-20240320-00096 |
| 中文关键词: 超高剂量率 电子线 秀丽线虫 发育 寿命 |
| 英文关键词:Ultra-high dose rate Electron beams Caenorhabditis elegans Development Life span |
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| 中文摘要: |
| 目的 利用模式生物-秀丽隐杆线虫研究超高剂量率脉冲电子线对机体的影响。方法 将成年期野生型株系(N2)线虫同步化后培养至L4期,随机分为对照组(SHAM组)、常规剂量率组(CONV组)和超高剂量率组(UHDR组)。CONV组和UHDR组辐射剂量均为3 Gy(脉冲电子线照射,剂量率分别为0.3和200 Gy/s)。辐照结束后,计数每组产卵量;照后3、6和10 d时,分别检测线虫发育、运动能力和存活时间。结果 照后第3天时,CONV组和UHDR组线虫体长均明显短于SHAM组(t=4.81、4.83,P<0.05),体宽差异无统计 学意义(P>0.05)。第6和10天时,与SHAM组相比,CONV组体长和体宽均明显减小(t=3.18~ 3.63,P<0.05);而UHDR组体长明显增加(t=-9.85、-2.87,P<0.05),体宽差异无统计学意义(P>0.05)。与CONV组比较,第6和10天时,UHDR组体宽明显增加(t=-4.43、-3.37,P<0.05)。与SHAM组相比,照后第6天,CONV组和UHDR组头部摆动、身体弯曲次数均明显下降(t=2.91、3.52、3.97、2.71,P<0.05);第10天,3组运动能力均较弱且差异均无统计学意义(P>0.05)。与SHAM组相比,CONV组和UHDR组产卵量均显著降低(t=1.72、5.54,P<0.05),但UHDR组线虫产卵量显著高于CONV组(t=-5.99,P<0.05)。与SHAM组相比,CONV组线虫寿命明显缩短(χ2=8.49,P<0.05),而UHDR组线虫存活时间差异无统计学意义(P>0.05)。结论 CONV电子射线可导致秀丽线虫发育受损、运动能力降低、产卵量减少和寿命缩短,但在相同剂量下UHDR脉冲电子射线引起的损伤程度较轻。 |
| 英文摘要: |
| Objective To explore the effects of ultra-high dose rate pulsed electron beams on Caenorhabditis elegans (C. elegans). Methods The adult wild-type strain (N2) of C. elegans was synchronized and cultured to L4 stage, and then randomly divided into control group (SHAM group), conventional radiotherapy dose rate group (CONV group) and ultra-high dose rate radiation group (UHDR group). The CONV and UHDR groups were exposed to 3 Gy of the pulsed electron beam at dose rates of 0.3 and 200 Gy/s, respectively. After irradiation, the egg-laying capacity of each group was assessed, and the developmental progress, motility, and survival rates each were evaluated at day 3, 6, and 10. Results On the 3rd day post-irradiation, both the CONV and UHDR groups showed shorter body lengths compared to the SHAM group (t=4.81, 4.83, P<0.05), with no significant differences in body width (P>0.05). On the 6th and 10th days, the CONV group showed a significant reduction in both body length and width compared to the SHAM group (t=3.18-3.63, P<0.05), whereas the UHDR group displayed a significant increase in body length (t=-9.85, -2.87, P<0.05) with no significant change in body width. When comparing the UHDR group to the CONV group on day 6 and 10, a significant increase in body width was observed (t=-4.43, -3.37,P<0.05). Motor activity, including head swinging and body bending, significantly decreased in the CONV and UHDR groups compared to the SHAM group on day 6 (t=2.91, 3.52, 3.97, 2.71, P<0.05), with no significant differences among the three groups by day 10 (P>0.05). Egg-laying capacity significantly reduced in both irradiated groups compared to the SHAM group (t=1.72, 5.54, P<0.05), while the UHDR group exhibited higher fecundity than the CONV group (t=-5.99, P<0.05). Lifespan was significantly shortened in the CONV group compared to the SHAM group (χ2=8.49, P<0.05), whereas the survival time of the UHDR group was not significantly differ from that of the SHAM group (P>0.05). Conclusions Exposure to a conventional electron beam result in developmental delays, reduced mobility, decreased fecundity, and a shortened lifespan in C. elegans. However, only slight side effects were observed when C. elegans were exposed to an ultra-high dose rate pulsed electron beam at the same dosage. |
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