| 田庄,何琪,乔璇,李林涛,胡瑄,全晓筱,叶彤,甘慧,吴卓娜,顾若兰,朱晓霞,孙云波,刘曙晨,窦桂芳,孟志云.放射性核素污染伤口去污膜的研制与表征[J].中华放射医学与防护杂志,2024,44(4):247-254 |
| 放射性核素污染伤口去污膜的研制与表征 |
| Development and characterization of a decontamination film for radionuclide-contaminated wound |
| 投稿时间：2023-11-02 |
| DOI：10.3760/cma.j.cn112271-20231121-00181 |
| 中文关键词: 洗消剂 去污膜 放射性污染 |
| 英文关键词:Chelating agent Decontamination film Radioactive contamination |
| 基金项目: |
| 作者 | 单位 | E-mail | | 田庄 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 何琪 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 乔璇 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 李林涛 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 胡瑄 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 全晓筱 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 叶彤 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 甘慧 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 吴卓娜 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 顾若兰 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 朱晓霞 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 孙云波 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 刘曙晨 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 窦桂芳 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | | | 孟志云 | 军事科学院军事医学研究院辐射医学研究所, 北京 100850 | mengzhiyun@vip.163.com |
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| 中文摘要: |
| 目的 研发一款适用于放射性核素污染的去污膜,对其物理性质、核素清除效果和安全性进行考察。方法 以海藻酸钠和壳聚糖为基质膜,负载复方洗消剂配方制备去污膜。通过将去污膜置于生理盐水中,测试其溶胀性能;通过拉力机测试去污膜的断裂应力与应变。以生理盐水、基质膜、1%二乙烯三胺五乙酸五钠(DTPA-5Na) 基质膜组作为对照组,在豚鼠完整皮肤与伤口模型上检测去污膜对铀、铯、钴、铈和锶的清除效果。采用噻唑蓝比色法 (MTT)测试去污膜的浸提液对小鼠上皮样成纤维细胞 (L929) 的毒性;将去污膜的浸提液注射到 Kunming (KM) 小鼠体内,测试其全身急性毒性。将去污膜覆盖于新西兰兔背部测试皮肤刺激性。结果 去污膜成膜时间为 1.5 min,溶胀率为 (134.96 ± 3.49)%,断裂应力为 (393.88 ± 53.53)kN/m2, 断裂应变为 (163.00 ± 35.29)%。在完整皮肤上,去污膜对铀的清除率为 (95.38 ± 0.23)%,对铯为 (96.57 ± 0.49)%。在伤口模型上,去污膜对铀、铯、锶、钴和铈的清除率分别为(92.16 ± 0.52)%、(90.44 ± 1.16)%、(92.03 ± 0.87)%、(92.79 ± 0.51)% 和(92.85 ± 0.82)%,均优于生理盐水、基质膜与1% DTPA-5Na基质膜组 (t = 3.81 ~ 4 498.55,P<0.001)。安全性评价试验表明,去污膜符合国家医疗器械生物学评价标准。结论 去污膜能够快速成膜且对多种核素均有良好的清除效果,安全性良好,适用放射性核素污染人员的完整皮肤或伤口去污,有望为核污染区域的工作人员提供安全保障。 |
| 英文摘要: |
| Objective To develop a decontamination film for radionuclide contamination and to examine its physical properties, nuclide removal effect, and safety. Method The decontamination film was prepared using sodium alginate and chitosan as matrix film, where a chelating agent formulation was loaded. The dissolution properties of the decontamination film were determined by placing it into normal saline. Its fracture stress and strain were measured using a tensile machine. A DTPA-5Na-based matrix film group, prepared using saline, matrix film, and 1% diethylenetriaminepentaacetic acid pentasodium salt (DTPA-5Na), served as the control group. Then, the effects of the decontamination film on the removal of uranium, cesium, cobalt, cerium, and strontium were examined on the intact skin and wound model of guinea pigs. A colorimetric assay-the methyl thiazolyl tetrazolium (MTT) assay-was employed to determine the toxicity of the decontamination film's leach liquor for clone 929 (L929) fibroblasts. The decontamination film's leach liquor was injected into Kunming (KM) mice to test its acute systemic toxicity. The skin irritation of the decontamination film was determined by covering of the film on the backs of New Zealand rabbits. Results The decontamination film exhibited a formation time of 1.5 min, dissolution rates of (134.96±3.49)%, fracture stress of (393.88±53.53) kN/m2, and fracture strain of (163.00%±35.29)%. The decontamination film removed (95.38±0.23)% of uranium and (96.57±0.49)% of cesium from the intact skin of guinea pigs. Furthermore, it removed (92.16±0.52)% of uranium, (90.44±1.16)% of cesium, (92.03±0.87)% of strontium, (92.79±0.51)% of cobalt, and (92.85±0.82)% of cerium from the wound model, with the decontamination rates all higher than those of normal saline and the 1% DTPA-5Na-based matrix film group (t = 3.81 - 4 498.55, P < 0.001). The safety evaluation tests indicated that the decontamination film met the national standard for the biological evaluation of medical devices. Conclusions The decontamination film, which can be formed swiftly and effectively remove a variety of nuclides with high safety, is suitable for the decontamination of the intact skin or wounds of personnel subjected to radionuclide contamination. It is expected that the decontamination film will provide a safety barrier for staff working in nuclear-contaminated areas. |
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