| 陈婕,刘萌萌,高玲.基于γ-H2AX焦点检测的局部照射剂量估算三维模型方法的建立[J].中华放射医学与防护杂志,2024,44(3):188-193.Chen Jie,Liu Mengmeng,Gao Ling.Establishing a three-dimensional model for estimating partial-body irradiation doses based on γ-H2AX foci detection[J].Chin J Radiol Med Prot,2024,44(3):188-193 |
| 基于γ-H2AX焦点检测的局部照射剂量估算三维模型方法的建立 |
| Establishing a three-dimensional model for estimating partial-body irradiation doses based on γ-H2AX foci detection |
| 投稿时间：2023-09-11 |
| DOI：10.3760/cma.j.cn112271-20230911-00083 |
| 中文关键词: γ-H2AX 局部照射 剂量估算 三维模型 |
| 英文关键词:γ-H2AX Partial-body irradiation Dose estimation Three-dimensional model |
| 基金项目:北京市自然科学基金(7162139,7202137);国家自然科学基金(82273551,31570852);中国疾病预防控制中心辐射防护与核安全医学所青年科学研究所长基金(2020-04) |
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| 中文摘要: |
| 目的 构建基于γ-H2AX焦点检测的时间-剂量-效应三维剂量估算模型,并对其可行性进行验证。方法 按随机数表法将小鼠分为0 、2 、4、6、8 Gy组,每组3只,进行X射线全身照射。照后1、6、24 h取胡须毛囊细胞。免疫荧光染色后,利用激光共聚焦显微镜观察照后1~24 h不同时间点γ-H2AX焦点数。将观察到的γ-H2AX平均焦点数经Dolphin's模型校正后,拟合剂量-效应关系曲线。使用R软件,利用照射剂量、照后时间和校正后的γ-H2AX平均焦点数进行局部照射三维模型方程和曲面的建立。结果 γ-H2AX平均焦点数在固定时间点1、6和24 h随剂量的增加而增加,但在固定剂量点2、4、6、8 Gy随照射时间的延长而减少。拟合的局部照 射剂量-效应关系曲线方程为:照后1 h,YF = 2.853+3.775 D,R2= 0.928;照后6 h,YF = 0.144+ 2.775 D,R2= 0.903;照后24 h,YF = 0.066+2.472 D,R2= 0.85。拟合的三维模型方程为YF = 6.837 t-1.728+3.113 t-0.071 D,R2 = 0.897。将不同的照后时间代入三维曲面模型后呈现二维线性模型形式。将γ-H2AX焦点数和照射时间代入线性模型和三维模型表明,使用线性模型和三维模型估算受照剂量与实际照射剂量的相对偏差均不超过30%。结论 使用γ-H2AX焦点数进行局部照射剂量估算,建立了时间-剂量-效应三维模型,此模型可初步用于照后1~24 h内所有时间点的剂量估算。 |
| 英文摘要: |
| Objective To construct a three-dimensional time-dose-response model for dose estimation and validate its feasibility. Methods Based on a random number table, mice were divided into 0, 2, 4, 6, and 8 Gy groups for whole-body X-ray irradiation, with each group consisting of three mice. Hair follicle cells of whiskers were sampled at 1, 6, and 24 h after the irradiation. After immunofluorescence staining, the numbers of γ-H2AX foci at different time points from 1 to 24 h post-irradiation were observed using a confocal laser scanning microscope. The average numbers of γ-H2AX foci observed were corrected using the Dolphin’s model, followed by the fitting of dose-response curves. Using the R software, the equations and surfaces of the three-dimensional model for partial-body irradiation were established using the irradiation doses, post-irradiation time, and the corrected average numbers of γ-H2AX foci. Results The average number of γ-H2AX foci increased with dose at fixed time points 1, 6, and 24 h but decreased with irradiation time at fixed doses 2, 4, 6, and 8 Gy. The dose-response curve equations of partial-body irradiation were fitted as follows: YF = 2.853+3.775 D, R2= 0.928, at 1 h after the irradiation; YF = 0.144+2.775 D, R2= 0.903, at 6 h after the irradiation; YF = 0.066+2.472 D, R2= 0.85, at 24 h after the irradiation. The three-dimensional model equation fitted was YF = 6.837 t-1.728+3.113 t-0.071 D, R2=0.897. Substituting different post-irradiation time points into the three-dimensional surface model appeared as a two-dimensional linear model. By substituting the number of γ-H2AX foci and irradiation time into the linear and the three-dimensional models, both models yielded relative deviations between the estimated and actual radiation doses of 30% or less. Conclusions The three-dimensional time-dose-response model, established by using the number of γ-H2AX foci to estimate partial-body irradiation doses, can be preliminarily applied for dose estimation at all time points 1-24 h after irradiation. |
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