| 罗辉,马蕾杰,毛荣虎,等.超高剂量率照射减轻斑马鱼胚胎放射损伤的作用及机制研究[J].中华放射医学与防护杂志,2024,44(3):174-180.Luo Hui,Ma Leijie,Mao Ronghu,et al.Effects and mechanism of ultra-high dose rate irradiation in reducing radiation damage to zebrafish embryos[J].Chin J Radiol Med Prot,2024,44(3):174-180 |
| 超高剂量率照射减轻斑马鱼胚胎放射损伤的作用及机制研究 |
| Effects and mechanism of ultra-high dose rate irradiation in reducing radiation damage to zebrafish embryos |
| 投稿时间：2023-10-23 |
| DOI：10.3760/cma.j.cn112271-20231023-00135 |
| 中文关键词: 超高剂量率照射 斑马鱼 胚胎 氧化应激 放射损伤 |
| 英文关键词:Ultra-high dose rate irradiation Zebrafish Embryo Oxidative stress Radiation damage |
| 基金项目:河南省科技攻关计划项目(212102310619);河南省医学科技攻关计划项目(LHGJ20210171) |
| 作者 | 单位 | E-mail | | 罗辉 | 郑州大学附属肿瘤医院肿瘤放疗科, 郑州 450008 | | | 马蕾杰 | 郑州大学附属肿瘤医院肿瘤放疗科, 郑州 450008 | | | 毛荣虎 | 郑州大学附属肿瘤医院肿瘤放疗科, 郑州 450008 | | | 李娜 | 山东第一医科大学 山东省医学科学院放射学院, 泰安 271021 | | | 薛福敏 | 郑州大学附属儿童医院, 郑州 450018 | | | 王雪楠 | 山东第一医科大学 山东省医学科学院放射学院, 泰安 271021 | | | 雷宏昌 | 郑州大学附属肿瘤医院肿瘤放疗科, 郑州 450008 | | | 宋帅 | 郑州大学附属肿瘤医院肿瘤放疗科, 郑州 450008 | | | 葛红 |
1 郑州大学附属肿瘤医院肿瘤放疗科, 郑州 450008
2 山东第一医科大学 山东省医学科学院放射学院, 泰安 271021
3 郑州大学附属儿童医院, 郑州 450018
| zlyygehong0199@zzu.edu.cn | | 杨成梁 |
1 郑州大学附属肿瘤医院肿瘤放疗科, 郑州 450008
2 山东第一医科大学 山东省医学科学院放射学院, 泰安 271021
3 郑州大学附属儿童医院, 郑州 450018
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| 中文摘要: |
| 目的 对比分析超高剂量率(FLASH)照射和常规剂量率照射后斑马鱼胚胎的放射损伤及其产生的生物学机制。方法 以受精后4 h的斑马鱼胚胎为研究对象,分别实施常规剂量率照射和FLASH照射(9 MeV电子线),统计射线暴露后斑马鱼的死亡率和孵化率。对照射后96 h的幼鱼形态评分,检测活性氧(ROS)含量,分析幼鱼体内氧化应激相关指标的变化。结果 尽管2~12 Gy电子束照射对斑马鱼胚胎死亡率及孵化率无显著作用,但单次大剂量照射(≥6 Gy)可导致幼鱼发育畸形,以常规照射最为明显(t=0.87~9.75,P<0.05)。FLASH照射(≥6 Gy)后,斑马鱼体内ROS及氧化应激相关指标超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)较常规照射显著降低(t=0.42~15.19,P<0.05)。常规照射和FLASH照射后孵育液内ROS含量差异无统计学意义(P>0.05)。结论 FLASH照射后斑马鱼胚胎的放射损伤较常规照射明显减轻,呈现出剂量依赖性。两种照射模式后斑马鱼氧化应激水平存在差异,这可能是FLASH照射放射损伤较轻的重要因素。 |
| 英文摘要: |
| Objective To conduct a comparative analysis of the radiation damage to zebrafish embryos and the associated biological mechanism after ultra-high dose rate (FLASH) and conventional dose rate irradiation. Methods Zebrafish embryos at 4 h post-fertilization were exposed to conventional and FLASH irradiation (9 MeV electron beam). The mortality and hatchability of zebrafish after radiation exposure were recorded. Larvae at 96 h post-irradiation underwent morphological scoring, testing of reactive oxygen species (ROS) levels, and analysis of changes in oxidative stress indicators. Results Electron beam irradiation at doses of 2-12 Gy exerted subtle effects on the mortality and hatchability of zebrafish embryos. However, single high-dose irradiation (≥ 6 Gy) could lead to developmental malformation of larvae, with conventional irradiation showing the most significant effects (t = 0.87-9.75, P < 0.05). In contrast, after FLASH irradiation (≥ 6 Gy), the ROS levels in zebrafish and its oxidative stress indicators including superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were significantly reduced (t = 0.42-15.19, P < 0.05). There was no statistically significant difference in ROS levels in incubating solutions after conventional and FLASH irradiation (P > 0.05). Conclusions Compared to conventional irradiation, FLASH irradiation can reduce radiation damage to zebrafish embryos, and this is in a dose-dependent manner. The two irradiation modes lead to different oxidative stress levels in zebrafish, which might be a significant factor in the reduction of radiation damage with FLASH irradiation. |
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