| 李科君,杜利清,刘强,宋会娟.糖基纳米颗粒对辐射诱导的早期肺组织巨噬细胞极化的影响研究[J].中华放射医学与防护杂志,2023,43(11):858-865 |
| 糖基纳米颗粒对辐射诱导的早期肺组织巨噬细胞极化的影响研究 |
| Effects of glycosylated nanoparticles on radiation-induced the polarization of macrophages in early injured lung tissue |
| 投稿时间：2023-02-20 |
| DOI：10.3760/cma.j.cn112271-20230220-00172 |
| 中文关键词: 放射性肺损伤 肺巨噬细胞 M2极化 活性氧 炎症 |
| 英文关键词:Radiation-induced lung injury Pulmonary macrophages M2 polarization Reactive oxygen species Inflammation |
| 基金项目:国家自然科学基金(82001954,32071241,81972976,32171239);辐射防护实验室开放基金(CIRP-DTRI20220202);中国医学科学院医疗健康科技创新项目(2022-I2M-2-003) |
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| 中文摘要: |
| 目的 探究一种糖基纳米颗粒对辐射诱导的早期肺组织巨噬细胞极化以及活性氧清除的效果。方法 将20只小鼠按随机数表法分为对照组、给药组、照射组和照射+给药组。照射组和照射 + 给药组进行X射线全肺照射。通过流式细胞仪与聚合酶链式反应(PCR)技术检测肺组织巨噬细胞的极化比例,利用PCR与酶联免疫吸附实验(ELISA)检测炎症因子表达水平,通过2',7'-二氯二氢荧光素的氧化双乙酸酯(DCFH-DA)荧光探针检测肺组织中活性氧(ROS)水平。结果 相比于照射组,照射+治疗组的ROS荧光信号强度明显降低(t=15.76,P<0.05)。同时,照射+治疗组的肺组织中M2型巨噬细胞比例显著提高(t=2.89,P < 0.05),且精氨酸酶1(ARG-1)基因表达水平升高,肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和白介素6(IL-6)炎症因子的表达水平显著降低(t=3.32、2.90、2.85、4.55、2.88,P < 0.05)。结论 糖基纳米颗粒能够有效促进辐射诱导的肺组织巨噬细胞向M2表型极化,同时能有效清除辐照区域的ROS,降低辐照组织的炎症水平。 |
| 英文摘要: |
| Objective To explore the effects of glycosylated nanoparticles on early radiation responses including the generation of reactive oxygen species (ROS), the polarization of pulmonary macrophages, and secretion of inflammatory cytokines in mice lung tissues.Methods Twenty mice were randomly divided into control group, drug administration group, irradiation group and irradiation + drug administration group. The irradiation group and irradiation + drug administration group were subjected to whole lung irradiation with X-rays. The antioxidant ability of glycosylated nanoparticles was characterized using ROS indicator (CM-H2DCFDA). The M2 polarization of pulmonary macrophages was detected by flow cytometry and PCR. The mRNA and protein expression levels of inflammatory cytokines were investigated by PCR and ELISA assay, respectively.Results Compared with the irradiation group, the intensity of ROS fluorescence signals was significantly lower (t=15.76, P < 0.05), the proportion of M2-type macrophages was significantly higher (t=2.89, P < 0.05), the expression level of arginase 1 (ARG-1) gene was elevated, and the expression levels of tumour necrosis factor α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) inflammatory factors were significantly reduced (t=3.32, 2.90, 2.85, 4.55, 2.88, P < 0.05) in the lung tissues of irradiation + treatment group.Conclusions Glycosylated nanoparticles can effectively scavenge ROS, trigger polarization of M2 macrophage, dampen inflammatory responses, and thus potentially alleviate radiation-induced lung injury. |
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