| 唐菁,马骥祥,李志媛,李杰,张鹏程.基于DNA双链断裂损伤簇的相对生物效能模拟计算[J].中华放射医学与防护杂志,2023,43(7):489-498 |
| 基于DNA双链断裂损伤簇的相对生物效能模拟计算 |
| Simulation calculation of relative biological effectiveness based on DNA double strand break clusters |
| 投稿时间：2023-02-20 |
| DOI：10.3760/cma.j.cn112271-20230220-00039 |
| 中文关键词: DNA双链断裂损伤簇|相对生物效能|含噪声密度聚类算法 |
| 英文关键词:DNA double strand break clusters|Relative biological effectiveness|Density-based spatial clustering of applications with noise algorithm |
| 基金项目:国家自然科学基金(11605160) |
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| 中文摘要: |
| 目的 从追溯辐射引起的DNA损伤机制出发,根据基本的生物物理原理获取DNA双链断裂(DSB)损伤簇表示的相对生物效能(RBE)值,探讨DNA损伤与染色体畸变、生殖细胞死亡生物效能的相似性。方法 以低能电子、质子、α粒子作为研究对象,通过辐射物理化学模型,模拟细胞核受粒子辐射的过程。在基于损伤点密度的含噪声密度聚类(DBSCAN)算法的基础上,改进DSB损伤簇分类 方法,以减弱模拟过程中损伤点与能量沉积随机分布假设的联系,使得DSB损伤簇更接近于非随机分布。计算获得DSB损伤簇的产额,提出以DSB损伤簇计算RBE值的方法。结果 2 MeV α粒子的DSB损伤簇RBE值(12.29)与生物实验中染色体片段(15.3±5.9)、细胞存活(14.7±5.1)表示的RBE相近。结论 高传能线密度(LET)电离辐射后,不同于简单DSB,DSB损伤簇的RBE与染色体畸变、细胞存活生物效能存在一定的相似性。 |
| 英文摘要: |
| Objective To obtain the relative biological effectiveness (RBE) of DNA double strand breaks (DSB) clusters by tracing the mechanism of radiated DNA damage, and explore the relationship among the biological effectiveness of DNA damage, chromosomal aberrations and germ cell death. Methods Taking low-energy electrons, protons, and α particles as the research objects, this study simulated the process that cell nuclei were exposed to particle radiation using a radiation-related physicochemical model. On the ground of the DSB density-based spatial clustering of applications with noise (DBSCAN) algorithm, the DSB cluster classification method was improved to weaken the connection between the DSBs and the random distribution assumptions of energy depositions during the simulation. In this manner, the DSB clusters can be much closer to a non-random distribution. Furthermore, this study obtained the yields of DSB clusters and proposed a method to calculate the RBE values of DSB clusters. Results The calculated RBE value (12.29) of DSB clusters of 2 MeV α particles was similar to the experimental RBE values of chromosomal fragments (15.3±5.9) and cell survival (14.7±5.1). Conclusions After high-LET ionizing radiation, unlike the single DSB, the RBE of DSB clusters was similar to that of chromosomal aberration and cell survival. |
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