| 周桂强,薛一哲,张兆文,秦佟洲,郭玲,来盼盼,高鹏,王星,黄志飞,臧宇航,荆云涛,丁桂荣.5G手机通信频段射频辐射对小鼠血脑屏障的影响[J].中华放射医学与防护杂志,2023,43(3):176-181 |
| 5G手机通信频段射频辐射对小鼠血脑屏障的影响 |
| Effect of radiofrequency radiation from 5G mobile phone on blood-brain barrier in mice |
| 投稿时间：2022-10-25 |
| DOI：10.3760/cma.j.cn112271-20221025-00418 |
| 中文关键词: 5G手机 射频辐射 小鼠 血脑屏障 |
| 英文关键词:5G mobile phone Radio frequency radiation Mice Blood brain barrier |
| 基金项目:空军军医大学专项课题（2021HKYX08） |
| 作者 | 单位 | E-mail | | 周桂强 | 潍坊医学院公共卫生学院, 潍坊 261053 | | | 薛一哲 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 张兆文 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 秦佟洲 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 郭玲 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 来盼盼 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 高鹏 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 王星 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 黄志飞 | 潍坊医学院公共卫生学院, 潍坊 261053 | | | 臧宇航 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 荆云涛 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | | | 丁桂荣 | 空军军医大学军事预防医学系辐射防护医学教研室, 西安 710032 | dingzhao@fmmu.edu.cn |
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| 中文摘要: |
| 目的 探讨5G手机通讯频段（3.5和4.9 GHz）射频辐射（RF）对小鼠血脑屏障通透性的影响。方法 将24只成年雄性C57BL/6小鼠（6~8周龄）按照随机数表法分为假照射组（Sham）、3.5 GHz RF组和4.9 GHz RF组，每组8只。RF组小鼠连续35 d （1 h/d）全身暴露于功率密度为50 W/m2的5G手机辐射。采用EB荧光实验检测小鼠血脑屏障通透性的改变。Western blot用于检测血脑屏障紧密连接相关蛋白ZO-1、Occludin、Claudin-11和缝隙连接相关蛋白Connexin 43的表达水平。结果 与Sham组相比，3.5 GHz RF组和4.9 GHz RF组小鼠大脑皮层EB光斑数量和荧光强度明显增加，荧光综合得分增加，差异有统计学意义（t=12.98、17.82，P<0.001）。ELISA结果显示，3.5 GHz RF组和4.9 GHz RF组小鼠血清S100B含量显著增加（t=19.34、14.68，P<0.001）。Western blot结果显示，与Sham组相比，3.5 GHz RF组小鼠大脑皮层Occludin蛋白水平均显著降低（t=-3.13，P<0.05），且4.9 GHz RF组变化更明显（t=-6.55，P<0.01）。两个RF组小鼠大脑皮层ZO-1、Claudin-11和Connexin 43蛋白水平较Sham组无明显变化（P>0.05）。结论 3.5 GHz或4.9 GHz射频辐射连续照射小鼠35 d （1 h/d），可诱导小鼠大脑皮层血脑屏障通透性增加，其机制可能与5G手机通信频段射频辐射导致的大脑皮层紧密连接相关蛋白Occludin减少有关。 |
| 英文摘要: |
| Objective To investigate the effect of radiofrequency radiation (RF) from 5G mobile phone communication frequency bands (3.5 GHz and 4.9 GHz) on the permeability of the blood-brain barrier (BBB) in mice.Methods A total of 24 healthy adult male C57BL/6 mice (6-8 weeks old) were randomly divided into Sham, 3.5 GHz RF and 4.9 GHz RF groups, and 8 mice in each group. Mice in the RF groups were systemically exposed to 5G cell phone radiation for consecutive 35 d(1 h/d) with 50 W/m2 power density. The BBB permeability of mice was detected by Evans Blue (EB) fluorescence experiment. The expression levels of the BBB tight junction-related proteins (ZO-1, occludin and claudin-11) and the gap junction-related protein Connexin 43 were determined by Western blot.Results The number of spots, fluorescence intensity and comprehensive score of EB were significantly increased in 3.5 GHz RF group and 4.9 GHz RF group compared with the Sham group (t=12.98, 17.82, P < 0.001). Compared with the Sham group, the content of S100B in mouse serum was significantly increased in 3.5 GHz RF group and 4.9 GHz RF group (t=19.34, 14.68, P < 0.001). The BBB permeability was increased in the RF group. The expression level of occludin protein was significantly reduced in the 3.5 GHz RF group (t=-3.13, P < 0.05), and this decrease was much profound in the 4.9 GHz RF group (t=-6.55, P < 0.01). But the protein levels of ZO-1, Claudin-11 and Connexin 43 in the cerebral cortex of the RF groups had no significantly difference in comparison with the Sham group(P > 0.05).Conclusions The continuous exposure of mobile phone RF at 3.5 GHz or 4.9 GHz for 35 d (1 h/d) induces an increase of BBB permeability in the mouse cerebral cortex, perhaps by reducing the expression of occludin protein. |
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