| 廖聆郦,杨帆,马雨薇,王璐瑶,屈振,王效静,江浩,周咏春.电离辐射通过诱导外泌体的分泌促进宫颈癌Siha细胞上皮间质转化[J].中华放射医学与防护杂志,2022,42(12):922-927 |
| 电离辐射通过诱导外泌体的分泌促进宫颈癌Siha细胞上皮间质转化 |
| Ionizing radiation promotes epithelial-mesenchymal transition of cervical cancer cell line Siha through increasing the secretion of exosomes |
| 投稿时间：2022-09-08 |
| DOI：10.3760/cma.j.cn112271-20220908-00366 |
| 中文关键词: 宫颈癌|电离辐射|上皮间质转化|外泌体 |
| 英文关键词:Cervical cancer|Ionizing radiation|Epithelial-mesenchymal transition|Exosomes |
| 基金项目:国家自然科学基金(31200633);安徽省自然科学基金面上项目(2008085MH245);蚌埠医学院"512人才培育计划"项目(BY51201211);蚌埠医学院研究生科研创新计划项目(Byycx21093) |
|
| 摘要点击次数: 1242 |
| 全文下载次数: 404 |
| 中文摘要: |
| 目的 观察宫颈癌Siha细胞在临床常规分割放疗模式的电离辐射诱导后上皮间质转化(EMT)的发生情况,以及外泌体在此过程中的作用。方法 利用临床常规分割外照射(6 MV-X射线,50 Gy/25次)模式的电离辐射处理宫颈鳞癌细胞系Siha,通过形态学观察、EMT标志物检测和Transwell小室实验评价Siha细胞EMT的发生;分离纯化细胞上清液中外泌体,利用透射电镜、纳米粒径示踪分析(NTA),观察其形态与分泌量;最后加入抑制剂GW4869(10 μmol/L)明确外泌体作用。结果 经电离辐射处理后,存活的Siha细胞发生了EMT现象:梭形间质样细胞出现,上皮标志物E-cadherin表达下调(t=9.66,P<0.05),间质标志物N-cadherin表达上调(t=41.61,P<0.05),Transwell穿膜和穿透基质胶的细胞数目增多(t=6.11、13.22,P<0.05)。此外,辐射诱导后,Siha细胞外泌体分泌增加(t=7.51,P<0.05),而GW4869负性调控后,其细胞外泌体的分泌随之减少(t=7.28,P<0.05),电离辐射诱导的EMT也发生了逆转。结论 临床常规分割放疗模式的电离辐射可通过诱导外泌体的分泌促进宫颈癌Siha细胞上皮间质转化。 |
| 英文摘要: |
| Objective To observe the occurrence of epithelial-mesenchymal transition (EMT) in cervical cancer cell line Siha irradiated by X-rays with clinical conventional fraction radiotherapy model and investigate the role of exosomes in this process.Methods Siha cells were irradiated by 6 MV-X rays with 50 Gy in 25 fractions. EMT was evaluated by cell morphology, EMT biomarkers and cell migration and invasion ability. Exosomes released from cells were detected by transmission electron microscopy and nanoparticle tracking analysis (NTA), and its function in EMT was explored by using an exosome inhibitor GW4869 (10 μmol/L).Results After irradiation, EMT phenomenon was induced in the survived Siha cells, including the incidence of mesenchymal phenotype, upregulation of epithelial marker E-cadherin (t=9.66, P<0.05), downregulation of mesenchymal marker N-cadherin (t=41.61, P<0.05), and increase of cell migration and invasion abilities (t=6.11, 13.22; P<0.05). Meanwhile, the secretion of exosomes was also increased after irradiation (t=7.51, P0.05). When the cells were pre-treated with GW4869, radiation-induced exosome secretion was reduced (t=7.28, P0.05), so that radiation-induced EMT was reversed.Conclusions Ionizing radiation with clinical conventional fraction radiotherapy model promotes EMT of cervical cancer cells through increasing the secretion of exosomes. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|