| 刘国利,宋秀军,王叶梅,余祖胤,申星,时磊,金花,王欣茹.巴特日-7对小鼠放射性肠损伤的防治作用研究[J].中华放射医学与防护杂志,2022,42(11):839-844 |
| 巴特日-7对小鼠放射性肠损伤的防治作用研究 |
| Preventive and therapeutic effects of Bateri-7 on radiation-induced intestinal injury in mice |
| 投稿时间：2022-06-30 |
| DOI：10.3760/cma.j.cn112271-20220630-00275 |
| 中文关键词: 蒙药 巴特日-7 放射性肠损伤 放射性肠炎 |
| 英文关键词:Mongolian medicine Bateri-7 Radiation-induced intestinal injury Radiation enteritis |
| 基金项目:内蒙古民族大学科学研究项目(NMDYB20067) |
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| 中文摘要: |
| 目的 探讨蒙药巴特日-7对12 Gy 60Co γ射线全身照射小鼠肠道辐射损伤的防护作用。方法 C57BL/6J雄性小鼠按随机数表法分为对照组、照射组和照射给药组,每组15只。存活实验分为照射组和照射给药组,每组10只。12 Gy 60Co γ射线全身单次照射,对照组和照射组给予生理盐水灌胃,照射给药组给予巴特日-7(530 mg/kg)灌胃,给药方式为照射前7 d和照射后3 d连续给药。照射后6和24 h免疫组织化学法检测Tunel阳性细胞;照射后3.5 d HE染色观察肠道绒毛结构,免疫组织化学法检测BrdU、Ki67阳性细胞;实时荧光定量PCR(qPCR)检测小肠白介素6(IL-6)、肿瘤坏死因子α(TNF-α)和趋化因子5(Cxcl-5)表达水平;测定外周血异硫氰酸荧光素-葡聚糖(FITC-dextran)含量。结果 照射给药组小鼠存活时间长于照射组(χ2= 5.84,P < 0.05),小鼠体重变化两组间差异无统计学意义(P> 0.05);照射给药组小肠绒毛长度明显大于照射组(t = 20.24,P < 0.05),两组小肠隐窝深度比较差异无统计学意义(P> 0.05)。照射后6和24 h,照射给药组肠隐窝Tunel阳性细胞数量较照射组明显减少(t = 3.52、2.90,P< 0.05)。照射后3.5 d,照射给药组小鼠血清FITC-dextran水平、小肠组织IL-6、TNF-α和Cxcl-5的表达均明显低于照射组(t = 6.92、7.01、7.18、13.16,P< 0.05);而BrdU、Ki67阳性隐窝数高于照射组(t =3.91、2.57,P< 0.05)。结论 蒙药巴特日-7能够有效改善小鼠辐射后肠道损伤,对放射性肠炎具有较好的防治作用,为药物治疗放射性肠炎提供新的依据。 |
| 英文摘要: |
| Objective To study the protective effect of Mongolian medicine Bateri-7 on radiation-induced intestinal injury in mice.Methods C57BL/6J male mice were randomly divided into control group, irradiation group and irradiation plus drug administration group, with 10 or 15 mice in each group. For irradiation group, the mice were given a single dose of 12 Gy 60Co γ-rays with total body irradiation. For drug treatment, the mice were gavaged with Bateri-7 (530 mg/kg) 7 d before irradiation until 3 d after IR. At 6 h and 24 h after irradiation, the Tunel positive cells in intestine were detected immunohistochemically. At 3.5 d after irradiation, the structure of intestinal villi was observed by HE staining, and the BrdU and Ki67 positive cells were detected immunohistochemically. The expression levels of IL-6, TNF-α and Cxcl-5 were detected by qPCR. The FITC-dextran in peripheral blood was also determined.Results The survival of irradiated mice was significantly increased by Bateri-7 (χ2= 5.84, P < 0.05), but there was no significant difference in weight between two groups (P > 0.05). The villi length of small intestine in the irradiation plus drug group was significantly longer than that in the irradiation group (t = 20.24, P < 0.05), and there was no significant difference in the depth of intestinal crypt between two groups (P > 0.05). At 6 and 24 h after irradiation, the number of Tunel positive cells in intestinal crypts in the irradiation plus drug group was significantly reduced in comparison with the irradiation group (t = 3.52, 2.90, P < 0.05). At 3.5 d after irradiation, the level of FITC-dextran in serum and the expressions of IL-6, TNF-α and Cxcl-5 in small intestine of mice in the irradiation plus drug group were significantly lower than those in the irradiation group, respectively(t = 6.92, 7.01, 7.18, 13.16,P < 0.05). The number of BrdU and Ki67 positive cells in the crypt of mice in the irradiation plus drug group was higher than that of the irradiation group (t = 3.91, 2.57, P < 0.05).Conclusions Mongolian medicine Bateri-7 can effectively alleviate irradiation-induced intestinal injury of mice, which may have a good preventive and therapeutic effect on radiation enteritis. |
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