| 陈忠民,张蒙,徐龙江,李峰生,孙亮,陈秋,涂彧,崔凤梅.氚水诱发血管内皮细胞差异表达lncRNAs和mRNAs的筛选和初步功能分析[J].中华放射医学与防护杂志,2022,42(6):428-432 |
| 氚水诱发血管内皮细胞差异表达lncRNAs和mRNAs的筛选和初步功能分析 |
| Screening and preliminaryfunctional analysis of differentially expressed lncRNAs and mRNAs in vascular endothelial cells treated by tritiated water |
| 投稿时间：2022-01-11 |
| DOI：10.3760/cma.j.cn112271-20220111-00014 |
| 中文关键词: 氚水 血管内皮细胞 长链非编码核糖核酸 信使核糖核酸 |
| 英文关键词:Tritiated water Vascular endothelial cells lncRNAs mRNAs |
| 基金项目:国家自然科学基金核技术创新联合基金(U186720);军队后勤科研计划(BEP20J004) |
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| 中文摘要: |
| 目的 探讨氚水诱发人脐静脉血管内皮细胞(human umbilical vein endothelial cells，HUVEC)lncRNAs和mRNAs的表达改变及其意义。方法 HUVEC细胞分为两组，分别为对照组和氚水染毒组。对照组细胞用DMEM培养基培养，氚水染毒组细胞在含有氚水的培养基中培养(氚水终浓度为3.7×103 Bq/ml)。两组细胞均持续培养48 h后收集细胞样本，提取RNA，采用高通量芯片技术筛选差异表达lncRNAs和mRNAs，并进行数据分析。结果 氚水染毒组与对照组相比，lncRNAs分析显示有1 717个lncRNA显著上调，3 994个lncRNA显著下调；mRNAs分析显示，有4 562个基因显著上调，1 433个基因显著下调。差异mRNA与差异lncRNA通过共表达分析，获取关键基因，包括SQSTM1、CXCL8、ITPR1、GADD45A、NF-kB1和VDAC1等基因。结论 氚水暴露可诱发血管内皮细胞发生多个mRNAs与lncRNAs的表达改变，可能通过SQSTM1、CXCL8和ITPR1等关键基因的信号通路导致毒性效应。 |
| 英文摘要: |
| Objective To investigate the expression changes of lncRNAs and mRNAs in human umbilical vein endothelial cells(HUVEC) treated by tritiated water.Methods HUVEC cells were divided into two groups, the control group cultured in DMEM medium, and the tritiated water exposure group cultured in a medium containing tritiated water with a final concentraion of 3.7×103 Bq/ml. After culture for 48 h, cells were collected for RNA extract.The differentially expressed lncRNAs and mRNAs were screened by high-through put chip technology and then analyzed.Results Compared with the control group, 1 717 lncRNAs were significantly up-regulated and 3 994 lncRNAs significantly down-regulated, and 4 562 mRNAs were significantly up-regulated and 1 433 mRNAs down-regulated. Through co-expression analysis of differential mRNAs and lncRNAs, some key genes including SQSTM1, CXCL8, ITPR1, GADD45A, NF-kB1 and VDAC1 were obtained.Conclusions Tritiated water exposure can induce multiple changes of mRNAs and lncRNAs in vascular endothelial cells, which may lead to toxic effects through signaling pathways including some key genes such as SQSTM1, CXCL8, and ITPR1. |
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