| 赵骅,习聪,刘海翔,等.60Co γ射线全身均匀照射大鼠早期血浆辐射敏感脂质筛选研究[J].中华放射医学与防护杂志,2022,42(3):175-180.Zhao Hua,Xi Cong,Liu Haixiang,et al.Screening of radiation sensitive lipids in rat plasma at early stage after total body irradiation with 60Co γ-rays[J].Chin J Radiol Med Prot,2022,42(3):175-180 |
| 60Co γ射线全身均匀照射大鼠早期血浆辐射敏感脂质筛选研究 |
| Screening of radiation sensitive lipids in rat plasma at early stage after total body irradiation with 60Co γ-rays |
| 投稿时间：2021-09-02 |
| DOI：10.3760/cma.j.cn112271-20210902-00354 |
| 中文关键词: 脂质组学 电离辐射 生物标志物 受试者工作特征曲线 剂量-效应 |
| 英文关键词:Lipidomics Ionizing radiation Biomarker Receiver operating characteristic curve Dose-response |
| 基金项目:国家自然科学基金（82003393，82173463） |
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| 中文摘要: |
| 目的 探索大鼠受到全身照射后血浆脂质代谢特征，为辐射生物标志物研究提供科学依据。方法 非靶向脂质组学研究中，将50只SD大鼠分为6组，用0、1、2、3、5、8 Gy钴60 γ射线进行全身照射；靶向脂质组学研究中，将25只大鼠分为5组，用0、0.5、2.5、4、6 Gy射线进行照射。照射后4 h，采集静脉血并分离血浆，筛选辐射敏感脂质并测定其浓度，进行受试者工作特征曲线（ROC）和剂量-效应关系分析。结果 非靶向脂质组学研究中，共筛选出15个辐射差异脂质；经靶向脂质组学验证，其中7个可作为辐射敏感脂质。辐射敏感脂质ROC区分0 Gy组与> 0 Gy组、< 2 Gy组与≥ 2 Gy组样本曲线下面积（AUC）均> 0.75；将其组合后进行ROC分析，AUC值提高为0.96和0.94。在0~6 Gy范围内，LysoPC（18：2）、LysoPC（22：0）、PC（18：0/18：2）、PE（18：2/16：0）和PE（18：2/18：0）浓度随照射剂量的上升而下降。结论 大鼠受照后4 h，共筛选出7个血浆辐射敏感脂质，其组合可用于特定照射剂量分类，其中5个脂质具有良好的剂量-效应关系。 |
| 英文摘要: |
| Objective To explore the characteristics of lipid metabolism in rat plasma after total body irradiation(TBI) in order to provide scientific evidence of radiation biomarkers. Methods For the non-targeted lipidomics study, 50 SD rats were divided into 6 groups and irradiated with 0, 1, 2, 3, 5 or 8 Gy 60Co γ-rays, respectively. For the targeted lipidomics study, 25 rats were divided into 5 groups and irradiated with 0, 0.5, 2.5, 4 or 6 Gy. Venous blood samples were collected and plasma were separated 4 h after TBI. Radiation-sensitive lipids were screened and their concentrations were determined. Receiver operating characteristic curve (ROC) and dose-response were analyzed. Results A total of 15 radiation differential lipids were screened out based on non-targeted lipidomics study and 7 of them were identified as radiosensitive lipids by targeted lipidomics analysis. The ROC of radiosensitive lipids distinguished area under curve (AUC) of samples in 0 Gy group and > 0 Gy group, < 2 Gy group and ≥ 2 Gy group were all > 0.75. The AUC values were increased to 0.96 and 0.94 after the panel of radiation sensitive lipids ROC analysis. The concentrations of LysoPC(18: 2), LysoPC(22: 0), PC(18: 0/18: 2), PE(18: 2/16: 0) and PE(18: 2/18: 0) decreased with irradiation dose within 0-6 Gy. A total of 7 plasma radiosensitive lipids in rat plasma were identified 4 h after TBI, and the panel of them could be used for specific dose classification. Five of the lipids had good dose-response relationship. |
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