| 马娅,侯殿俊,郭磊,宋丹,朱伟,李卫国,毛雪松,方连英,王仲文.儿童脉管性疾病患者介入手术后外周血淋巴细胞染色体畸变观察[J].中华放射医学与防护杂志,2022,42(2):89-94 |
| 儿童脉管性疾病患者介入手术后外周血淋巴细胞染色体畸变观察 |
| Observation on chromosome aberration of peripheral blood lymphocytes in children with vascular diseases after interventional procedure |
| 投稿时间：2021-09-22 |
| DOI：10.3760/cma.j.cn112271-20210922-00387 |
| 中文关键词: 电离辐射 介入手术 儿童 双着丝粒染色体 |
| 英文关键词:Ionizing radiation Interventional procedure Children Dicentric chromosome |
| 基金项目:国家自然科学基金青年科学基金项目（32100999）；山东省医药卫生科技发展计划项目（202012070680）；山东省自然科学基金项目（ZR2021MH053，ZR2020QC082） |
|
| 摘要点击次数: 2411 |
| 全文下载次数: 698 |
| 中文摘要: |
| 目的 评估儿童脉管性疾病患者在介入手术过程中接受的电离辐射（IR）对患儿染色体畸变的影响。方法 在知情同意的条件下，于介入术前和术后立刻采集26名患儿的外周血1.2 ml，培养制备染色体，分析外周血淋巴细胞染色体畸变情况。结果 儿童介入术后双着丝粒染色体+着丝粒环（dic+r）率明显增加，与术前比较，差异有统计学意义（U=647，P<0.01）。术后dic+r率与年龄、体重、累积剂量面积乘积（DAP）、累积剂量（CD）及估算的有效剂量（E）之间并无相关性（P>0.05），不同性别之间、不同年龄组之间及不同的病灶部位之间，术后dic+r率差异无统计学意义（P>0.05），4名患儿的术后dic+r率超过40×10-3，其中有1例高达95.83×10-3。结论 脉管性疾病患儿在介入术中接受的IR可导致dic+r率增加且存在个体差异，部分儿童异常敏感。儿童介入手术的正当化与防护工作需引起高度重视。 |
| 英文摘要: |
| Objective To evaluate the effect of ionizing radiation on chromosome aberration of peripheral blood lymphocytes in children with vascular interventional procedure. Methods The chromosome aberration of peripheral blood lymphocytes was analyzed before and after vascular interventional procedure to assess the effect of low-dose ionizing radiation on chromosomes. Peripheral blood (PB) was collected using heparin as an anticoagulant from 26 patients before and after (within 10 minutes) vascular interventional surgery. Informed consent was obtained from the patients' parents. Results Dicentric + centric ring (dic+r) formation increased significantly (U=647, P<0.01) in peripheral blood lymphocytes of children after interventional operation. No correlation was observed between the rate of dic+r and the cumulative dose (CD), dose area product (DAP), age, body weight, gender and the estimated effective dose E (P>0.05). There was no significant difference in dic+r rate of children between different genders, ages and lesion sites (P>0.05). The dic+r rate after interventional operation exceeded 40×10-3 in 4 of 26 cases with the highest of 95.83×10-3. Conclusions The ionizing radiation during children's vascular interventional procedure might lead to the increase of chromosome aberration of peripheral blood lymphocytes and some children are abnormally sensitive. Great attention should be paid to the justification and radiation protection during interventional procedure for children. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |