| 马丽萍,刘青杰,田雨汀,刘方,田梅,高玲.褪黑素通过调控p53介导的细胞早衰抑制紫外线诱导的HaCaT细胞中黑色素合成[J].中华放射医学与防护杂志,2021,41(12):920-925 |
| 褪黑素通过调控p53介导的细胞早衰抑制紫外线诱导的HaCaT细胞中黑色素合成 |
| Melatonin inhibits UVB-induced melanin synthesis by regulating p53-mediated premature senescence in HaCaT cells |
| 投稿时间：2021-07-20 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.12.007 |
| 中文关键词: 褪黑素 黑色素合成 细胞早衰 p53 TYR |
| 英文关键词:Melatonin Melanin synthesis Premature senescence p53 TYR |
| 基金项目:北京市自然科学基金（7162139，7202137）；国家自然科学基金（31570852） |
|
| 摘要点击次数: 1914 |
| 全文下载次数: 754 |
| 中文摘要: |
| 目的 探索褪黑素对中波紫外线（UVB）引起人永生化表皮角质形成（HaCaT）细胞黑色素合成的影响及机制，为褪黑素的皮肤保护机制提供理论基础。方法 80 mJ/cm2 UVB照射10-5 mol/L褪黑素预处理的HaCaT细胞，照后48和72 h，利用NaOH法检测细胞黑色素水平。照后72 h，利用β-半乳糖苷酶染色试剂盒检测早衰阳性细胞并分析其比例，蛋白免疫印迹法检测衰老相关蛋白p53和酪氨酸酶（TYR）的表达变化。80 mJ/cm2 UVB照射分别经毛细血管扩张性共济失调突变激酶/毛细血管扩张性共济失调Rad3相关激酶（ATM/ATR）抑制剂、p53抑制剂和褪黑素预处理的HaCaT细胞，照后72 h检测细胞早衰阳性比例和黑色素水平变化。结果 褪黑素抑制UVB诱导的黑色素水平增加（t=56.65、13.39，P<0.05），同时抑制UVB诱导的TYR表达增加（t=16.46，P<0.05）；并可缓解UVB诱导的HaCaT细胞早衰（t=6.37，P<0.05），抑制UVB诱导的p53表达增加（t=19.08，P<0.05）；ATM/ATR抑制剂、p53抑制剂和褪黑素预处理均可抑制UVB诱导的HaCaT细胞黑色素水平升高（t=13.88、7.86、8.96，P<0.05）。结论 褪黑素通过调控p53介导的细胞早衰，抑制UVB诱导的HaCaT细胞黑色素水平增加。 |
| 英文摘要: |
| Objective To explore the mechanism and regulatory effects of melatonin on UVB-induced melanin synthesis in human immortalized keratinocytes (HaCaT), so as to provide a theoretical basis for the skin protection of melatonin. Methods HaCaT cells were pretreated with 10-5 mol/L melatonin and then irradiated with 80 mJ/cm2UVB. The melanin content was detected by NaOH assay, the proportion of premature senescence cells was detected by β-galactosidase staining kit, and the protein expression levels of both p53 and tyrosinase (TYR) were detected by Western blot at 72 h after UVB exposure. After 12 h pretreatment of ATM/ATR inhibitor, p53 inhibitor and melatonin, the proportion of premature senescence and the change of melanin content in HaCaT cells were detected at 72 h after 80 mJ/cm2 UVB irradiation. Results Melatonin inhibited UVB-induced increases of melanin content (t=56.65, 13.39, P<0.05) and TYR expression (t=16.46, P<0.05) in HaCaT cells. Melatonin alleviated UVB-induced premature senescence (t=7.139, P<0.05) and inhibited UVB-induced increase of p53 expression (t=19.08, P<0.05) in HaCaT cells. In addition, ATM/ATR inhibitor, p53 inhibitor and melatonin all inhibited UVB-induced increase of melanin content in HaCaT cells. Conclusions Melatonin inhibits TYR-mediated melanin synthesis by regulating p53-related premature senescence in HaCaT cells after UVB irradiation. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |