| 王宁,屈东昊,林宇涵,等.α-硫辛酸对小鼠耳蜗带状突触的辐射保护作用[J].中华放射医学与防护杂志,2021,41(8):609-614.Wang Ning,Qu Donghao,Lin Yuhan,et al.Protective effect of α-lipoic acid on cochlear ribbon synapses in mice injured by ionizing radiation[J].Chin J Radiol Med Prot,2021,41(8):609-614 |
| α-硫辛酸对小鼠耳蜗带状突触的辐射保护作用 |
| Protective effect of α-lipoic acid on cochlear ribbon synapses in mice injured by ionizing radiation |
| 投稿时间：2020-01-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.08.008 |
| 中文关键词: α-硫辛酸 电离辐射 带状突触 耳蜗 |
| 英文关键词:Alpha-lipoic acid Ionizing radiation Ribbon synapses Cochlear |
| 基金项目:辽宁省自然科学基金（2015020342） |
|
| 摘要点击次数: 5375 |
| 全文下载次数: 1755 |
| 中文摘要: |
| 目的 观察电离辐射对小鼠耳蜗带状突触损伤情况，并探讨α-硫辛酸（LA）对其的保护作用。方法 随机区组法将小鼠分为健康对照组、照射3 d组、照射3 d+LA组、照射14 d组和照射14 d+LA组，每组10只。各照射组使用辐照仪进行16 Gy X射线照射；照射+LA组，照射后给予LA每日1次；健康对照组给予等量生理盐水。所有小鼠在照射和处死前行听性脑干反应（ABR）测试。免疫荧光标记蛋白ctBP2，观察带状突触数量变化。Western blot对蛋白otoferlin、AP-2表达水平进行半定量分析。结果 与健康对照组相比，照射组ABR阈值显著增高（P<0.05），且照射14 d组最高（P<0.05）；照射+LA组阈值则明显降低（P<0.05）；照射3 d组和照射14 d组，12、24 kHz分别与8 kHz阈移比较，差异均无统计学意义（P>0.05）；32与8 kHz阈移比较显著增高（t=-2.38、-5.48，P<0.05）。各组带状突触数量，照射组较健康对照组明显减少（P<0.05），且照射14 d组最少（P<0.05）；照射+LA组则明显增多（P<0.05）。AP-2和otoferlin蛋白水平，照射组较健康对照组明显减低（P<0.05），且照射后14 d组更低（P<0.05）；照射+LA组则明显升高（P<0.05）。结论 LA对耳蜗内毛细胞带状突触的损伤具有保护作用。 |
| 英文摘要: |
| Objective To explore the protective effect of α-lipoic acid (LA) on radiation damage of mice cochlear ribbon synapses.Methods Mice were divided into five groups:control group, radiation 3 d group, radiation 3 d+LA group, radiation 14 d group and radiation 14 d+LA group. The radiation groups were irradiated with 16 Gy, the radiation+LA groups were given LA once a day after radiation, the control group was given the same amount of normal saline. The auditory brainstem response (ABR) of mice were measured before irradiation and sacrifice. The number of ribbon synapses were observed with immunofluorescently labeled protein ctBP2. Western blot assay was performed to obtain the semi-quantitative expression levels of otoferlin and AP-2 protein.Results Compared with the control group, the ABR threshold of radiation groups were significantly higher (P<0.05) with the highest value at 14 d after irradiation (P<0.05), and the ABR threshold of the radiation+LA groups were significantly lower (P<0.05). The ABR threshold shifts of 12 kHz, 24 kHz at 3 d and 14 d groups had no significant difference with 8 kHz threshold shift (P>0.05). The 32 kHz threshold shift was significantly higher than 8 kHz threshold shift (t=-2.38, -5.48, P<0.05). The number of ribbon synapses in the radiation groups was significantly lower than that of control group (P<0.05), with the lowest value in the radiation 14 d group. LA treatment increased the ABR value significantly (P<0.05). AP-2 and otoferlin protein levels were significantly reduced after irradiation, especially in the radiation 14 d groups, and they were increased by the LA treatment.Conclusions LA has protective effect on the ribbon synapses of cochlear hair cells. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |