| 吴涵旭,王嘉豪,蒋雅雯,等.全胸照射引起的大鼠血浆辐射损伤早期代谢特征研究[J].中华放射医学与防护杂志,2020,40(12):919-925.Wu Hanxu,Wang Jiahao,Jiang Yawen,et al.Early metabolic characterization in plasma of rats exposed to whole thorax irradiation[J].Chin J Radiol Med Prot,2020,40(12):919-925 |
| 全胸照射引起的大鼠血浆辐射损伤早期代谢特征研究 |
| Early metabolic characterization in plasma of rats exposed to whole thorax irradiation |
| 投稿时间：2019-12-23 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.12.005 |
| 中文关键词: 电离辐射 代谢组学 气相色谱-质谱联用(GC-MS) 辐射损伤 |
| 英文关键词:Ionizing radiation Metabonomics Gas chromatography-mass spectrometry (GC-MS) Radiation injury |
| 基金项目:国家自然科学基金（81773361，U1967220） |
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| 中文摘要: |
| 目的 探索大鼠全胸照射（WTI）后早期血浆代谢特征的变化，为寻找非均匀局部照射辐射损伤生物标志物以及揭示辐射损伤机制提供实验依据。方法 雄性Wistar大鼠按完全随机分组法分为健康对照组和WTI照射组。气相色谱-质谱联用（GC-MS）技术考察大鼠WTI后第2、3、5天血浆代谢物水平的动态变化，筛选出辐射损伤特征差异代谢物，并对其涉及的代谢通路进行探讨。结果 主成分分析（PCA）显示健康对照组和WTI照射组的代谢差异随照后时间的延长愈加明显。与健康对照组相比，WTI后3~5 d，丙氨酸、棕榈酸、硬脂酸、乳酸、葡萄糖等代谢物在血浆中显著性上升（F=13.51、5.20、9.87、10.59、7.05，P<0.05），蛋氨酸、缬氨酸、苏氨酸、富马酸、苹果酸、α-酮戊二酸、磷酸、赖氨酸等显著性下降（F=15.68、5.63、3.78、12.83、2.88、8.93、4.68、6.43，P<0.05）。结论 WTI照射可引起血浆代谢轮廓改变，差异代谢物主要涉及氨基酸代谢、三羧酸（TCA）循环、脂类代谢等代谢途径。 |
| 英文摘要: |
| Objective Investigate the early metabolic characterization in plasma of rats exposed to whole thorax irradiation (WTI) in order to disclose the early biomarkers of non-uniform local irradiation and provide the experimental evidence of the mechanism of radiation injury. Methods Male Wistar rats were randomly divided into healthy control group and irradiation group. The dynamic alternations of metabolites in plasma of rats on the day 2, 3 and 5 after WTI were detected by gas chromatography mass spectrometry (GC-MS), and the differential metabolic features and metabolic pathway concerning radiation injury were analyzed. Results Principal Component Analysis (PCA) showed that the metabolic differences between control and WTI groups became more obvious along with the time after irradiation. Compared to the control, some metabolites such as inosine, alanine, palmitic acid, stearic acid, lactic acid and glucose were significantly increased during 3-5 days after WTI(F=13.51, 5.20, 9.87, 10.59, 7.05, P<0.05), while some metabolites such as methionine, valine, threonic acid, fumaric acid, malic acid, α-ketoglutaric acid, phosphoric acid and lysine were obviously decreased(F=15.68, 5.63, 3.78, 12.83, 2.88, 8.93, 4.68, 6.43,P<0.05). Conclusions The metabolic profile changes in rat plasma after WTI mainly include amino acids metabolism, tricarboxylic acid (TCA) cycle and lipid metabolism. |
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