| 芮刚,刘力源,林加金,郭玲,薛一哲,来盼盼,高鹏,邢俊玲,丁桂荣.5.8 GHz射频辐射对大鼠学习记忆和海马神经元突触可塑性的影响[J].中华放射医学与防护杂志,2020,40(6):427-433 |
| 5.8 GHz射频辐射对大鼠学习记忆和海马神经元突触可塑性的影响 |
| Effects of 5.8 GHz radiofrequency radiation on learning and memory along with synaptic plasticity of hippocampal neurons in rats |
| 投稿时间：2019-11-19 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.06.003 |
| 中文关键词: 射频辐射 学习记忆 海马区 突触可塑性 |
| 英文关键词:Radiofrequency Learning and memory Hippocampus Synaptic plasticity |
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| 中文摘要: |
| 目的 探讨5.8 GHz射频辐射（radiofrequency，RF）暴露对大鼠学习记忆和海马神经元突触可塑性的影响，为科学评价5.8 GHz RF的潜在健康危害提供理论和实验参考。方法 56只健康成年雄性Sprague-Dawley大鼠按随机数表法分为假暴露组（Sham）和射频暴露组（RF），每组28只，RF组每天暴露1 h，连续暴露15 d或30 d，频率为5.8 GHz，全身比吸收率为1.15 W/kg。采用Morris水迷宫检测大鼠学习记忆能力；Nissl染色观察大鼠海马组织结构及神经元数量；Golgi染色观察大鼠海马CA1区树突棘密度；Western blot检测海马组织内突触后致密蛋白PSD95、突触小泡蛋白Synaptophysin的水平；液相色谱-质谱联用仪检测海马组织内神经递质含量。结果 Morris水迷宫实验中，RF暴露15和30 d，Sham组与RF组大鼠的逃逸潜伏期、穿越平台次数、目标象限停留时间百分比及首次到达平台的潜伏期差异均无统计学意义（P>0.05）；Sham组和RF组海马区组织结构与神经元数量、CA1区树突棘密度（顶树突棘密度：15 d分别为5.10±0.20、4.89±0.24，30 d分别为4.58±0.27、4.49±0.24；基树突棘密度：15 d分别为4.81±0.17、4.79±0.34，30 d分别为4.20±0.27、4.22±0.17）、海马组织内PSD95及Synaptophysin表达水平及海马组织内多种神经递质含量均无明显变化（P>0.05）。结论 本实验条件下的5.8 GHz RF暴露对雄性大鼠空间学习记忆及海马神经元突触可塑性无影响。 |
| 英文摘要: |
| Objective To investigate the effect of 5.8 GHz radiofrequency (RF) radiation on learning and memory along with hippocampal synaptic plasticity in rats, in order to provide theoretical and experimental references for scientific evaluation of potential hazards of 5.8 GHz RF radiation. Methods A total of 56 healthy adult male Sprague-Dawley rats were randomly divided into sham exposure group (n=28) and RF exposure group (n=28). RF groups were exposed to 5.8 GHz RF for 1 h each day in 15 d or 30 d continuously, and the whole-body absorption rate was 1.15 W/kg. The learning and memory ability of rats was tested by Morris water maze (MWM). The hippocampal structure of rats was observed by Nissl stain. The density of dendritic spines in CA1 region of hippocampus was detected by Golgi stain. The expression of synaptic related protein (PSD95, Synaptophysin) in hippocampus was detected by Western blot. The level of hippocampal neurotransmitters was detected by liquid chromatography-mass spectrometry. Results In MWM experiments, at 15 d and 30 d after RF exposure, there was no statistically significant difference between sham group and RF group in the escape latency, frequency of crossing plateau, percentage of stay time in plateau quadrant and latency of first arrival to the plateau (P>0.05). Besides, the structure and the number of neurons in the hippocampus, the density of apical and basal dendritic spines of pyramidal neurons in the CA1 region (apical:5.10±0.20, 4.89±0.24, 4.58±0.27, 4.49±0.24, and basal:4.81±0.17, 4.79±0.34, 4.20±0.27, 4.22±0.17, named as Sham 15 d group, RF 15 d group, Sham 30 d group, RF 30 d group, respectively), the expression of PSD95 and Synaptophysin and the level of multiple kinds of neurotransmitters in the hippocampus had no significant changes (P>0.05). Conclusions In this study, 5.8 GHz RF radiation has no significant influence on the spatial learning and memory ability along with the synaptic plasticity of hippocampal neurons of rats. |
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