| 赵骅,田雪蕾,习聪,高玲,田梅,刘青杰.大鼠血浆辐射敏感脂质代谢物的筛选[J].中华放射医学与防护杂志,2020,40(6):420-426 |
| 大鼠血浆辐射敏感脂质代谢物的筛选 |
| Screening of radiosensitive lipid metabolites in rat plasma |
| 投稿时间：2020-01-15 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.06.002 |
| 中文关键词: 脂质组学 电离辐射 生物标志物 剂量-效应 代谢通路 |
| 英文关键词:Lipidomics Ionizing radiation Biomarker Dose-response Metabolic pathway |
| 基金项目:国家自然科学基金（81573081） |
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| 摘要点击次数: 1910 |
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| 中文摘要: |
| 目的 筛选大鼠血浆辐射敏感脂质代谢物并探索其代谢通路，为辐射损伤生物标志物研究提供科学依据。方法 用60Co γ射线对大鼠进行全身照射，照射剂量分别为0、1、3、5 Gy，采用基于液相色谱质谱串联平台的非靶向脂质组学方法，检测大鼠受照后血浆中脂质代谢物的变化。结果 大鼠受照后7 d，血浆中共有20个脂质代谢物相对含量发生显著变化，其中13个明显上调，7个明显下调。12个脂质代谢物具有良好的剂量-效应关系。结论 大鼠受到γ射线照射后，血浆中脂质代谢物水平发生显著变化，主要涉及鞘脂代谢、甘油磷脂代谢、糖基磷脂酰肌醇代谢等代谢通路。 |
| 英文摘要: |
| Objective To screen radiosensitive lipid metabolites in rat plasma and analyze their metabolic pathways in order to provide scientific basis for radiation damage biomarker. Methods The whole body irradiation of 60Co γ rays was performed to rats with different doses of 0, 1, 3 and 5 Gy. The changes of lipids in plasma were detected by untargeted lipidomics method based on liquid chromatography coupled mass spectrometry. Results Twenty plasma lipids were identified as the potential radiosensitive biomarkers at 7 days after irradiation, including 13 over-expressed lipids and 7 down-expressed lipids, where 12 lipids well responded to radiation doses. Conclusions Lipid metabolites in rat plasma are significantly changed after exposure to γ rays, and the metabolic pathways of sphingolipid, glycerophospholipid and glycosylphosphatidylinositol (GPI) are significantly enriched. |
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