中华放射医学与防护杂志

李爽,陆雪,陈德清,刘青杰.不同结构类型双着丝粒体建立的剂量-效应曲线估算剂量可行性研究[J].中华放射医学与防护杂志,2020,40(4):273-277

不同结构类型双着丝粒体建立的剂量-效应曲线估算剂量可行性研究

Study on the feasibility of dose estimation by the dose-response curves based on different types of dicentrics

投稿时间：2019-11-18

DOI：10.3760/cma.j.issn.0254-5098.2020.04.005

英文关键词:Chromosome aberration Dose-response curves Dicentric Ionizing radiation

基金项目:国家自然科学基金（81803162，81573081）

作者	单位	E-mail
李爽	中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088
陆雪	中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088
陈德清	中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088
刘青杰	中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088	liuqingjie@nirp.chinacdc.cn

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中文摘要:

目的探讨基于不同结构类型双着丝粒体（dicentrics，dic）建立的剂量-效应曲线估算生物剂量的可行性。方法采集两名健康人外周血样品，用0、0.5、1、2、3、4、5和6 Gy ⁶⁰Co γ射线（剂量率为0.27 Gy/min）离体照射人外周血，常规培养、收获和制备染色体标本，镜下分析并记录不同结构类型dic；应用CABAS软件建立dic剂量-效应曲线；并对两验证样本进行剂量估算。结果不同结构类型dic率均随受照剂量的增加而升高（R²=0.886~0.943，P<0.01），各剂量点经典型和单端型dic构成比之和约占所有类型dic的92%以上，而近距型dic和双端型dic分别在各照射剂量点的构成比均<4%。不同结构类型dic剂量-效应曲线的R²值均达到0.998；应用4条曲线估算的受照射剂量差异无统计学意义（P>0.05）。经典型dic剂量-效应曲线估算较高剂量时（3.9 Gy），相对偏差均≤13.08%。结论基于不同结构类型dic建立的剂量-效应曲线具有估算生物剂量的可行性。

英文摘要:

Objective To investigate the feasibility of dose estimation based on the dose-response curves established by different types of dicentrics (dic). Methods Human peripheral blood samples were irradiated by ⁶⁰Co γ-rays at the doses of 0, 0.5, 1, 2, 3, 4, 5 and 6 Gy with the dose rate of 0.27 Gy/min, and chromosome preparation was carried out. The different types of dics were analyzed. The dose-response curves were fitted with software of Chromosomal Aberration Calculation Software (CABAS). Two samples were used to validate the dose-response curves. Results The aberration rates of different types of dics increased with the exposure doses (R²=0.886-0.943,P<0.01).The proportion of typical and single-ended dic components accounted for more than 92% of all types of dic, while the proportion of short-range dic and double-ended dic components was less than 4% at each dose point. The dose-response curves based on different types of dics were established (R²=0.998, P<0.01). There was no significant difference in the doses estimated by four different dose-response curves (P>0.05). The relative deviations between actual and predict doses were less than 13.08% by using dose-response curve based on typical dics. Conclusions Dose-response curves based on different types of dics have the feasibility of dose estimation.

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