| 姚丹,张俊玲,郑旺,等.分割照射对肝癌移植瘤小鼠脾脏免疫细胞的影响[J].中华放射医学与防护杂志,2020,40(2):82-87.Yao Dan,Zhang Junling,Zheng Wang,et al.Effect of fractionated radiotherapy of transplanted hepatocellular carcinoma on the splenic immune cells in mice[J].Chin J Radiol Med Prot,2020,40(2):82-87 |
| 分割照射对肝癌移植瘤小鼠脾脏免疫细胞的影响 |
| Effect of fractionated radiotherapy of transplanted hepatocellular carcinoma on the splenic immune cells in mice |
| 投稿时间：2019-08-29 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.02.002 |
| 中文关键词: 肝癌 分割照射 脾脏淋巴细胞 |
| 英文关键词:Hepatocellular carcinoma Fractionated radiotherapy Splenic lymphocytes |
| 基金项目:国家重点研发计划项目（2017YFC0108604）；国家自然科学基金（31770910，31570850） |
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| 中文摘要: |
| 目的 探讨肝癌皮下移植瘤小鼠的分割照射对远端脾脏组织的影响。方法 构建荷瘤C57BL/6 J小鼠模型，取对数期生长的小鼠肝癌细胞Hepa 1-6接种于小鼠右侧皮下（1×107/只），荷瘤小鼠按随机数表法分为荷瘤对照组和照射组，每组20只。另设10只健康小鼠作为健康对照组。照射组皮下肿瘤给予8 Gy×3次X射线局部照射，剂量率为0.883 Gy/min。照射后第7、14天检测小鼠肿瘤脏器指数、脾脏脏器指数、脾脏病理学改变，以及脾脏T淋巴细胞亚群、B淋巴细胞亚群、NK细胞的变化。结果 与荷瘤对照组相比，照后7、14 d，照射组小鼠肿瘤脏器指数显著降低（t=4.649、26.34，P<0.05），脾脏中NK细胞显著升高（t=3.952、3.633，P<0.05），CD3+、CD4+、CD3+CD4+淋巴细胞以及CD4+/CD8+比值在照后7 d显著降低（t=3.193、3.656、3.219、2.641，P<0.05），CD3+淋巴细胞在照后14 d显著降低（t=3.031，P<0.05），其余指标脾脏脏器指数、B淋巴细胞、CD3+CD4+淋巴细胞、CD8+淋巴细胞变化不显著。结论 肿瘤局部照射可引起远隔器官内淋巴细胞比例失衡，导致机体免疫力下降，为放疗免疫损伤提供了新的诠释。 |
| 英文摘要: |
| Objective To investigate the effect of fractionated radiotherapy on the immune system of mice with subcutaneously transplanted hepatocellular carcinoma. Methods Logarithmic growth of mouse hepatocellular carcinoma cells Hepa 1-6 were inoculated subcutaneously on the right side of C57BL/6 J mice (1×107 cells/mice). The tumor-bearing mice were randomly divided into control group (Ctrl) and irradiation group (IR), 20 mice in each group. Additionally, 10 healthy mice were set as normal control group. Local fractionated X-ray irradiation of 8 Gy×3 fraction was given to the subcutaneous tumors, and the dose rate was 0.883 Gy/min. At 7 and 14 d after irradiation, the tumor organ index, spleen organ index, spleen pathological changes, and splenic T lymphocyte subsets, B lymphocyte subsets, and NK cells were detected. Results Compared with Ctrl, at 7 and 14 d after irradiation, the tumor organ index decreased (t=4.649, 26.34,P<0.05), and the percentage of NK cells increased significantly (t=3.952, 3.633,P<0.05). The percentages of CD3+, CD4+, CD3+CD4+ lymphocytes and the ratio of CD4+/CD8+ lymphocyte decreased at 7 d after irradiation (t=3.193, 3.656, 3.219, 2.641,P<0.05), and the percentage of CD3+lymphocyte decreased at 14 d after irradiation (t=3.031,P<0.05). But after irradiation, there were no significant changes in spleen organ index, B lymphocyte, CD3+ CD4+ lymphocyte and CD8+ lymphocyte. Conclusions Local hepatoma radiotherapy causes imbalance of lymphocytes in distal spleen of mice and hence reduces immunity, which provides a novel mechanism of radiological immunity damage. |
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