| 么一丹,张婷婷,胡凯,王仁生.肿节风在大鼠腮腺放射性损伤中的防护作用研究[J].中华放射医学与防护杂志,2020,40(1):11-18 |
| 肿节风在大鼠腮腺放射性损伤中的防护作用研究 |
| Protective effect of Sarcandra glabra on radiation-induced parotid injury in rats |
| 投稿时间：2019-07-29 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.01.002 |
| 中文关键词: 细胞凋亡 肿节风 放射性腮腺损伤 活性氧簇(ROS) 肿瘤坏死因子(TNF)-α |
| 英文关键词:Apoptosis Sarcandra glabra Radiation induced parotid injury ROS TNF-α |
| 基金项目:广西科学研究与技术开发项目（桂科合1599005-2-11）；中央引导地方科技发展专项（桂科ZY18076006） |
|
| 摘要点击次数: 1864 |
| 全文下载次数: 927 |
| 中文摘要: |
| 目的 研究经X射线照射后大鼠腮腺组织的炎症反应和细胞凋亡情况，探讨肿节风对放射性腮腺损伤的防护作用及可能的机制。方法 将120只雄性大鼠简单随机分成5组（每组24只）：对照组、单纯照射组、低（6.7 g·kg-1·d-1）、中（13.4 g·kg-1·d-1）、高（26.8 g·kg-1·d-1）剂量肿节风加照射组，给予单次15 Gy X射线照射大鼠腮腺组织，各组分别在照射后第10、40、70天将大鼠经2%戊巴比妥钠（0.16 ml/100 g）腹腔麻醉后经腹主动脉取血，检测血清中活性氧簇（ROS）含量，同时取腮腺组织用苏木精-伊红（HE）染色法和透射电镜观察其病理学改变和超微结构变化，应用免疫组织化学法检测腮腺组织中肿瘤坏死因子α（TNF-α）的表达水平，采用TUNEL法检测腮腺细胞的凋亡情况。结果 在同一时间点，单纯照射组中ROS的含量和TNF-α的表达较对照组明显升高（t=-24.723、-35.013、-19.515，P<0.05；t=-13.563、43.519、-15.249，P<0.05），而各药物组的上述观察指标介于二者之间，其中高剂量药物组低于低剂量药物组（t=5.295、8.138、6.545，P<0.05；t=10.093、-7.868、10.539，P<0.05）；对照组腮腺组织结构完好，无充血、渗出、水肿等；单纯照射组腮腺组织受照射后10 d出现充血、水肿、炎症细胞浸润，之后40 d时组织纤维化加重，各药物组腮腺组织的炎症反应明显减轻，且与药物剂量呈负相关。TUNEL结果同样显示单纯照射组腮腺细胞的凋亡率均较对照组增高（t=-4.639、-3.979，P<0.05）。结论 肿节风对放射性腮腺损伤具有一定的防护作用，其可能通过清除辐射产生ROS、减轻炎症反应和抑制细胞凋亡发挥作用，有望成为理想的放射性腮腺损伤防护剂。 |
| 英文摘要: |
| Objective To study the changes of inflammatory response and apoptosis in parotid gland tissues of rats after X-ray irradiation, and to explore the protective effect and possible mechanism of Sarcandra glabra on radiation-induced parotid injury in rats. Methods A total of 120 male rats were randomly divided into 5 groups(24 for each):control group, single irradiation group, radiation combined with a high(26.8 g·kg-1·d-1), moderate(13.4 g·kg-1·d-1) and low(6.7 g·kg-1·d-1) dosage of Sarcandra glabra group. The parotid gland of rats in the irradiation group received 15 Gy X-ray. Rats in each group were anesthetized with 2% pentobarbital sodium (0.16 ml/100 g) at 10, 40 and 70 d after irradiation and blood was collected from abdominal aorta. ROS levels in blood serum of each group were detected on the 10th, 40th and 70th days after irradiation. After parotid gland tissue was taken, the pathological changes and ultrastructural changes were observed by hematoxylin-eosin (HE) staining and transmission electron microscopy, respectively. The expression level of TNF-α in parotid gland tissue was detected by immunohistochemistry, and apoptosis of parotid cells was detected by TUNEL assay. Results The content of ROS and the expression of TNF-α protein in the single irradiation group were simultaneously increased compared with the control group (t=-24.723, -35.013, -19.515, P<0.05; t=-13.563, 43.519,-15.249, P<0.05), while they were reduced by Sarcandra glabra in a dosage dependent manner, especially in the high dosage group of Sarcandra glabra (t=5.295, 8.138, 6.545, P<0.05; t=10.093, -7.868, 10.539, P<0.05). In the control group, the parotid gland tissue structure was intact, without congestion, exudation, edema, etc. For the single irradiation group, the parotid gland tissue became hyperemia, edema and inflammatory cell infiltration at 10 d after irradiation followed by fibrosis at 40 d after irradiation. These pathological alterations in the parotid gland tissue were significantly recovered when the rats were treated with Sarcandra glabra before irradiation, and the tissue damage was negatively correlated with drug dosage. TUNEL assay showed that the apoptosis rate of parotid gland cells in the single irradiation groups was higher than that in the control group (t=-4.639, -3.979, P<0.05). Conclusions Sarcandra glabra protects parotid gland from radiation damage by scavenging radiation-induced ROS and declining inflammatory response, and thus it may be applied as a potential protective agent for radiation injury. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|