| 朱婉琦,贾丽,陈冠璇,李晓琳,邢力刚,赵汉玺,于金明.表没食子儿茶素没食子酸酯对放射性食管炎动物模型的作用研究[J].中华放射医学与防护杂志,2019,39(12):899-903 |
| 表没食子儿茶素没食子酸酯对放射性食管炎动物模型的作用研究 |
| Effect of epigallocatechin-3-gallate on radiation-induced esophagitis of rabbit |
| 投稿时间：2019-04-02 |
| DOI：10.3760/cma.j.issn.0254-5098.2019.12.004 |
| 中文关键词: 放射性食管炎 表没食子儿茶酸没食子酸酯 防护 |
| 英文关键词:Radiation injury Epigallocatechin-3-gallate Protection |
| 基金项目:浙江省省部共建项目（201339868） |
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| 摘要点击次数: 1957 |
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| 中文摘要: |
| 目的 研究表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate，EGCG）对放射性食管炎模型新西兰兔的影响。方法 取雄性新西兰兔30只，用随机数表法分为3组，每组10只，分别为空白组、生理盐水组、EGCG组。EGCG组、生理盐水组给予6 MV X射线照射造模，照射结束后分别给予EGCG和生理盐水治疗，每天3次，连续5 d。观察食管组织病理变化并评分，用酶联免疫吸附法测定血清白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、转化生长因子β（TGF-β）及肿瘤坏死因子α（TNF-α）水平，用免疫组织化学检测食管组织67LR的表达，应用Western blot检测67LR水平。结果 给药后，空白组、生理盐水组、EGCG组食管组织病理变化程度评分分别为0、3.90±1.10、2.80±0.92。3组血清IL-1β、IL-6、TGF-β及TNF-α水平的差异随着时间的延长显著增高（F=23.66~236.32，P<0.05）。3组食管组织67LR含量差异有统计学意义（F=585.38，P<0.05）。结论 EGCG能够减轻放射性食管炎的发生及严重程度，其作用可能与67LR表达有关。 |
| 英文摘要: |
| Objective To analyze the effect of epigallocatechin-3-gallate (EGCG) in radiation induced esophagitis of model rabbit. Methods Thirty male New Zealand rabbits were randomly divided into EGCG group,saline group,blank group. The rabbits in EGCG and saline groups were irradiated with 6 MV X-rays. The blank group did not receive radiation. After irradiation, rabbits were given with 440 μmol/L EGCG or saline three times a day in continuous 5 days. The scores of pathological changes of esophagus were observed by optical microscope.The serum levels of interleukine-1 beta (IL-1β), interleukine-6 (IL-6), transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay. The expression levels of 67KD laminin receptor (67LR) was detected by immunohistochemistry. Results After treatment, the scores of pathological changes of esophagus in blank group, saline group, EGCG group were 0, 3.9±1.10 and 2.80±0.92, respectively. At different time points after drug treatment, the levels of serum inflammatory factors among three groups were significantly different (F=23.66-236.32,P<0.05). The expressions of 67LR in esophageal tissue of 3 groups were also significantly different (F=585.38,P<0.05).Conclusions EGCG reduced radiation-induced esophagitis in rabbits by decreasing the levels of serum inflammatory factors, which may be related to the expression of 67LR protein. |
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