| 王永生,王明明.氚对神经细胞迁移相关因子表达及空间学习记忆能力的影响[J].中华放射医学与防护杂志,2019,39(12):887-892 |
| 氚对神经细胞迁移相关因子表达及空间学习记忆能力的影响 |
| Expression of neuronal migration-related factors and the spatial learning and memory of rats prenatally exposed to tritiated water |
| 投稿时间：2019-08-02 |
| DOI：10.3760/cma.j.issn.0254-5098.2019.12.002 |
| 中文关键词: 神经细胞 氚水 神经元迁移相关因子 空间学习与记忆能力 |
| 英文关键词:Neuronal cell Tritiated water Neuronal migration related factor Spatial learning and memory |
| 基金项目:安徽省自然科学基金（1208085MH162） |
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| 摘要点击次数: 1881 |
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| 中文摘要: |
| 目的 探讨氚水（HTO）对大鼠海马神经细胞迁移相关因子表达及空间学习记忆能力的影响。方法 取24 h内新生鼠脑海马细胞培养，培养第7天以0、3.7×102、3.7×103、3.7×104、3.7×105、3.7×106 Bq/ml终浓度氚水培养细胞24 h；Western blot法、RT-PCR法分别检测各浓度氚水处理后的神经细胞骨架蛋白（F-肌动蛋白、α-微管蛋白、tau蛋白及MAP2蛋白）、脑源性神经细胞营养因子（BDNF）及Reelin mRNA的表达情况。取妊娠14 d孕鼠16只，采用随机数表法分两组，每组8只，实验组单次注射3.7×106 Bq/g （体重）氚水，对照组注射等量体积生理盐水，Morris水迷宫法观察出生后两组大鼠的空间学习能力。结果 3.7×102、3.7×103、3.7×104、3.7×105、3.7×106 Bq/ml终浓度氚水处理后的神经细胞中，F-肌动蛋白（t=8.898~25.749，P<0.05）、α-微管蛋白（t=3.261~7.900，P<0.05）、tau （t=2.274~5.003，P<0.05）、MAP2（t=2.274~5.003，P<0.05）、BDNF mRNA （t=3.580~19.792，P<0.05）、Reelin mRNA （t=3.240~39.692，P<0.05）表达水平均低于0 Bq/ml浓度并随氚水浓度的升高表达量呈递减趋势。Morris水迷宫结果显示，与对照组相比，实验组大鼠逃避潜伏期延长（t=-2.563，P<0.05），目标象限穿行次数减少、停留时间缩短（t=3.214、3.874，P<0.05）。结论 HTO辐射对大鼠神经元迁移相关因子表达和空间学习有影响，这可能有助于了解放射性脑损伤的机制。 |
| 英文摘要: |
| Objective To study the expression of neuronal migration-related factors and spatial learning and memory of rats exposed to tritiated water (HTO). Methods Hippocampal neural cells from newborn Sprague-Dawley(SD) rats at postnatal 24 h were primarily cultured in DMEM/F12 medium with 20% of fetal bovine serum for 6 days, followed by subjection to tritiated water(HTO) at concentrations of 3.7×102, 3.7×103, 3.7×104, 3.7×105, 3.7×106 Bq/ml for 24 h, respectively. Western blot and RT-qPCR were used to determine the expression levels of F-actin, α-tubulin, tau, AP2, BDNF mRNA and Reelin mRNA. 16 pregnant SD rats at embryonic (E) day 14 were randomly divided into the tested and control groups (8 rats/each group). The tested rats were injected with body fluid of HTO (3.7×106 Bq/g) intraperitoneally, while the saline as the control. Morris water maze (MWM) was employed for the spatial learning and memory of rats. Results Compared to the control cells, HTO caused a significant downregulation of expressions of cytoskeletal proteins[F-actin (t=8.898-19.896, P<0.05), α-tubulin (t=3.261-7.900, P<0.05), tau (t=2.274-5.003, P<0.05), and MAP2 (t=2.274-5.003, P<0.05)] and mRNA of BDNF(t=3.580-19.792, P<0.05) and Reelin (t=3.240-39.692, P<0.05) in the tested neural cells in a dose-dependent manner. In addition, the escape latency of irradiated offsprings was significantly prolonged (t=-2.563, P<0.05), the time for offsprings to cross through target quadrant was markedly reduced (t=3.214, P<0.05), and the swimming time in the platform quadrant of irradiated offsprings were obviously shortened (t=3.874, P<0.05) in the MWM trial. Conclusions The results indicate that HTO irradiation in utero downregulates the expressions of neuron migration-related factors and induces brain dysfunction, which may shed a light on a mechanism of the radiation-induced brain impairment. |
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