| 张曲,徐姣珍,张弛,罗波,马建新.Stattic抑制STAT3和HIF-1α途径对食管癌裸鼠移植瘤放射敏感性的影响[J].中华放射医学与防护杂志,2018,38(11):815-818 |
| Stattic抑制STAT3和HIF-1α途径对食管癌裸鼠移植瘤放射敏感性的影响 |
| Radiosensitizion effect of stattic on the xenograft of ECA109 esophageal cancer cells in nude mice |
| 投稿时间：2018-06-26 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.11.003 |
| 中文关键词: Stattic 食管癌 放射敏感性 STAT3 缺氧诱导因子-1α |
| 英文关键词:Stattic ECA109 cells Radiosensitization STAT3 HIF-1α |
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| 中文摘要: |
| 目的 探讨Stattic对食管癌ECA109细胞裸鼠移植瘤的放射增敏效果及其可能的作用机制。方法 建立食管癌ECA109细胞裸鼠移植瘤模型；移植瘤平均体积增至约150 mm3时，采用随机数表法将24只裸鼠分为4组，药物+照射组（25 mg/kg Stattic+6 Gy）、单纯药物组（25 mg/kg Stattic）、单纯照射组（6 Gy）、对照组，每组6只。25 d后测量移植瘤体积，计算肿瘤体积抑制率，Western blot法检测移植瘤组织中STAT3、缺氧诱导因子-1α（HIF-1α）及血管内皮生长因子（VEGF）蛋白的表达。结果 药物+照射组裸鼠移植瘤体积为（705.1±75.5）mm3，显著低于单纯照射组（1 113.5±101.4） mm3和单纯药物组（1 696.5±100.6）mm3（t=4.35、14.14，P<0.05），其抑制率达（66.1±3.2）%。Western blot检测发现，药物+照射组移植瘤组织中pSTAT3、HIF-1α、VEGF蛋白表达水平较单纯照射组明显降低（t=17.07、5.05、3.54，P<0.05）。结论 Stattic对食管癌ECA109细胞裸鼠移植瘤具有放射增敏作用，可能与其抑制pSTAT3、HIF-1α、VEGF途径有关。 |
| 英文摘要: |
| Objective To investigate the radiosensitization effect of stattic on the xenograft of esophageal squamous cell carcinoma (ESCC) ECA109 cells in nude mouse and explore the underlying mechanisms. Methods Male nude mice inoculated subcutaneously with ECA109 cells were used to examine the radiosensitization effect of stattic in vivo. When average volume of tumors was about 150 mm3, mice were randomly divided into 4 groups:control group, 25 mg/kg stattic alone group, ionizing radiation (IR) (6 Gy) alone group, and 25 mg/kg stattic plus IR group. During the term of treatment, the volume of tumors was measured every 2 days. On 25 days after treatment, the mice were killed and the expressions of pSTAT3, STAT3, HIF-1α and VEGF in ECA109 xenografts were assessed by Western blot and immunohistochemistry analysis. Results The volume of tumor in nude mice was (705.1±75.5)mm3 in stattic plus IR group, which was significantly smaller than that in IR group (113.5±101.4)mm3 and stattic alone group(1 696.5±100.6)mm3 (t=4.35, 14.14,P<0.0). The inhibition rate was (66.1±3.2)% in stattic plus IR group. The expression levels of pSTAT3, HIF-1α and VEGF were obviously decreased in the stattic plus IR group compared with other groups (t=17.07, 5.05, 3.54, P<0.05). Conclusions Stattic play a radiosensitization role in the xenograft of esophageal carcinoma in nude mice probably by inhibiting the signaling pathways of pSTAT3, HIF-1α and VEGF. |
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