| 商军,解婷,殷娜瑞,陈飞,杜杰,张昊文,俞家华,刘芬菊.核定位序列修饰的二氧化钛纳米颗粒对胶质瘤U251细胞的放射增敏作用[J].中华放射医学与防护杂志,2018,38(11):801-806 |
| 核定位序列修饰的二氧化钛纳米颗粒对胶质瘤U251细胞的放射增敏作用 |
| Titanium dioxide nanoparticles coupled with nuclear localization sequence increases the radiosensitivity of U251 glioma cells |
| 投稿时间：2018-06-01 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.11.001 |
| 中文关键词: 二氧化钛纳米颗粒 核定位序列 放射增敏 胶质瘤细胞 |
| 英文关键词:Titanium dioxide nanoparticles Nuclear localization sequence Radiosensitivity Glioma cells |
| 基金项目:国家自然科学基金（31270897，81271682，31770913，81771947）；江苏省高校优势学科（PAPD） |
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| 中文摘要: |
| 目的 研究核定位序列（nuclear localization sequence，NLS）修饰的二氧化钛（titanium dioxide，TiO2）纳米颗粒对胶质瘤U251细胞放射敏感性的作用。方法 合成并表征具有细胞核靶向性的TiO2-NLS纳米颗粒，分别用纳米颗粒和电离辐射单独或联合处理U251细胞，流式细胞仪检测活性氧（ROS）含量和细胞凋亡率，γ-H2AX焦点（foci）染色检测DNA损伤修复的情况，细胞克隆形成实验检测细胞放射敏感性的改变。结果 NLS修饰可促进TiO2纳米颗粒进入U251细胞核，与未修饰的TiO2纳米颗粒相比，TiO2-NLS纳米颗粒可促进电离辐射诱导的细胞凋亡（t=8.96，P<0.05），细胞克隆实验显示，TiO2-BSA纳米颗粒组的放射增敏比为1.18，TiO2-NLS纳米颗粒处理组的放射增敏比为1.29，两组比较，差异有统计学意义（t=14.72，P<0.05）。结论 具有细胞核靶向性的TiO2纳米颗粒能够增强胶质瘤U251细胞的放射敏感性。 |
| 英文摘要: |
| Objective To investigate the effects of titanium dioxide (TiO2) nanoparticles coupled with nuclear localization sequence (NLS) on the radiosensitivity of U251 glioma cells. Methods Synthesis and characterization of the TiO2-NLS nanoparticles with nuclear targeting property. U251 cells were treated with nanoparticles and/or ionizing radiation. Flow cytometry analysis was performed to measure the ROS content and the cell apoptotic percentage. The DNA damage was detected by using γ-H2AX foci staining. Clonogenic survival assay was used to evaluate the radiosensitivity of U251 cells. Results NLS modification promoted nuclear translocation of TiO2nanoparticles. Compared with the control nanoparticles, TiO2-NLS treatment increased radiation-induced cell apoptosis (t=8.96, P<0.05). Clonogenic survival assay showed the radiosensitization ratios of TiO2 nanoparticle-treated group and TiO2-NLSnanoparticle-treated group were 1.18 and was 1.29, respectively. These two ratios had statistically difference (t=14.72, P<0.05). Conclusions Nuclear targeting TiO2 nanoparticles enhances the radiosensitivity of U251 glioma cells. |
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