| 宋延超,崔宏星,张庆召,侯长松,武云云.应用金纳米颗粒复合材料(ssDNA-AuNPs)比色检测γ射线照射剂量的可行性研究[J].中华放射医学与防护杂志,2018,38(8):612-616 |
| 应用金纳米颗粒复合材料(ssDNA-AuNPs)比色检测γ射线照射剂量的可行性研究 |
| The feasibility study of gold nanoparticle composites (ssDNA-AuNPs) for the colorimetric detection of gamma radiation dose |
| 投稿时间：2018-01-12 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.08.010 |
| 中文关键词: 金纳米颗粒 γ射线 剂量 比色检测 |
| 英文关键词:Gold nanoparticle γ-rays Radiation dose Colorimetric detection |
| 基金项目:国家自然科学基金（11375164） |
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| 摘要点击次数: 2389 |
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| 中文摘要: |
| 目的 建立检测γ射线照射剂量的新方法，探讨应用金纳米颗粒复合材料（ssDNA-AuNPs）比色检测γ射线照射剂量的可行性。方法 将寡核苷酸分子（ssDNA）修饰到金纳米颗粒（AuNPs）表面，制备出复合材料ssDNA-AuNPs。用60Co γ射线对其进行照射，剂量分别为0、5、10、20和30 Gy，而后观察溶液颜色变化并测量紫外-可见吸收光谱，建立吸收光谱中625 nm处与521 nm处吸光度的比值（A625/A521）与照射剂量线性关系。结果 随着60Co γ射线照射剂量的不断增加，ssDNA-AuNPs溶液的颜色由酒红色逐渐变为蓝紫色。在0~30 Gy剂量范围内，吸收光谱中A625/A521的比值对其拟合线性方程为A625/A521=0.020 6+0.303 6E（R2=0.991 5）。结论 本实验合成的ssDNA-AuNPs能够比色检测γ射线照射剂量，建立了一种比色检测γ射线照射剂量的新方法。 |
| 英文摘要: |
| Objective To establish a simple and convenient method and study the feasibility of using gold nanoparticle composites (ssDNA-AuNPs) for colorimetric detection of gamma radiation.Methods The composites ssDNA-AuNPs were prepared by applying the ssDNA to the surface of AuNPs, and then were irradiated with gamma ray with absorbed doses of 0, 5, 10, 20, and 30 Gy, respectively. Subsequently the color change in the solutions were observed simultaneously with absorption spectra being measured. The linear relationship between the ratio of A625/A521 in the absorption spectrum and the absorbed dose was established.Results With the increase of radiation dose, the color of the solutions changed from wine-red to blue-violet gradually, the ratio of A625/A521 in absorption spectra had excellent liner response for absorbed dose ranging from 0 to 30 Gy, and the linear equation was A625/A521=0.020 6+0.303 6 E(R2=0.991 5).Conclusions The ssDNA-AuNPs synthesized in this experiment can be successfully used for colorimetric detection of gamma absorbed dose, and a simple and convenient method for detection of gamma radiation has been newly established. |
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