| 张国军,杨川,黄建鸣,吕家华,李涛.葡萄糖耦联纳米金对A549肺癌细胞体外放射增敏作用[J].中华放射医学与防护杂志,2018,38(8):574-579 |
| 葡萄糖耦联纳米金对A549肺癌细胞体外放射增敏作用 |
| Radiosensitive effect of gold nanoparticles on lung cancer A549 cells |
| 投稿时间：2017-12-18 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.08.003 |
| 中文关键词: 纳米金 A549细胞 放射增敏作用 |
| 英文关键词:Gold nanoparticles A549 cells Radiosensitization effect |
| 基金项目: |
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| 中文摘要: |
| 目的 研究葡萄糖纳米金颗粒（Glu-GNPs）联合千伏和兆伏级X射线对肺腺癌A549细胞的体外放射增敏作用。方法 取指数生长期的人肺腺癌A549细胞，分为对照组、Glu-GNPs组、单纯照射组（6 MV单纯照射组与160 kV单纯照射组）、纳米金联合照射组（6 MV+Glu-GNPs组、160 kV+Glu-GNPs组）。使用透射电镜（TEM）观察Glu-GNPs在A549细胞中的分布。使用结晶紫测定法测定Glu-GNPs对A549细胞的毒性作用以及Glu-GNPs联合射线对细胞的增殖抑制作用。克隆形成实验评估Glu-GNPs对A549细胞的放射增敏作用。用γ-H2AX免疫荧光法检测A549细胞受照射后的DNA双链断裂焦点（foci）。结果 TEM显示Glu-GNPs主要分布在A549细胞质中，包括内涵体和线粒体内。浓度低于100 nmol/L的Glu-GNPs对A549细胞无明显毒性作用。不同浓度的Glu-GNPs联合不同能量级X射线对A549细胞均具有明显的增殖抑制作用。在160 kV与6 MV X射线条件下，Glu-GNPs联合照射组存活分数较单纯照射组明显降低（P<0.05），放射增敏比（SERD0）分别为1.41和1.15。此外Glu-GNPs可显著增加射线诱导的DNA双链断裂点，且Glu-GNPs联合160 kV X射线组与联合6 MV X射线组相比，有更多的DNA双链断裂灶点（t=12.392、14.893、18.947，P<0.05）。结论 Glu-GNPs可增加A549细胞的放射敏感性，且在keV条件下增敏效果更加明显。 |
| 英文摘要: |
| Objective To study the radiosensitization effect of thio-glucose capped gold nanoparticles (Glu-GNPs) on human lung adenocarcinoma A549 cells in vitro.Methods Human lung adenocarcinoma cell line A549 in logarithmic phase was divided into four groups:control group, drug group (Glu-GNPs), irradiation group (irradiation of 6 MV group and irradiation of 160 kV group), Glu-GNPs combined irradiation group (6 MV + Glu-GNPs group, 160 kV + Glu-GNPs group). Transmission electron microscopy (TEM) was used to observe the distribution of Glu-GNPs in cells. Toxicity of Glu-GNPs on A549 cells and the inhibitory effect of Glu-GNPs combined with irradiation on cell proliferation were determined using crystal violet assay. Clonogenic assay were performed to evaluate radiosensitization of Glu-GNPs on A549 cells. Immunofluorescence assay of γ-H2AX, a biomarker of DNA damage that underlies cellular response to irradiation was used to evaluate radiation-induced DNA double-strand break (DSB).Results TEM images showed that Glu-GNPs were mainly distributed in the cytoplasm of A549 cells, including endosomes and mitochondria. Glu-GNPs had little cytotoxicity toward A549 cells with a concentration lower than 100 nmol/L. Different concentrations(0-100 nmol/L)of Glu-GNPs combined with different energy of X-rays had significant inhibitory effects on A549 cells. Under 160 kV and 6 MV X-ray conditions, the Glu-GNPs treatment further decreased the survival fraction of irradiation group(P<0.05), and the sensitizing enhancement ratio (SER) was 1.41 and 1.15, respectively. Moreover, Glu-GNPs significantly increased radiation-induced γ-H2AX foci in A549 cells, and the number of γ-H2AX foci with 160 kV X-ray radiation was higher than that with 6 MV X-ray radiation(t=12.392, 14.893, 18.947, P<0.05).Conclusions Uptake of Glu-GNPs by A549 cells could enhance radiation effects, especially for kilovolt X-ray radiation. |
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