| 乃爱桃,王贞,曹文宇,徐杨,刘政海,何洁,陈熙,何淑雅,艾小红,万炜.丰富环境对辐射诱导小鼠认知障碍的保护作用及可能机制[J].中华放射医学与防护杂志,2018,38(6):401-406 |
| 丰富环境对辐射诱导小鼠认知障碍的保护作用及可能机制 |
| The protective effect of environmental enrichment on radiation induced cognitive dysfunction and underlying mechanism |
| 投稿时间：2018-01-12 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.06.001 |
| 中文关键词: 电离辐射 认知功能障碍 丰富环境 小胶质细胞激活 突触 |
| 英文关键词:Ionizing radiation Cognitive dysfunction Environmental enrichment Microglial activation Synapse |
| 基金项目: |
| 作者 | 单位 | E-mail | | 乃爱桃 | 421001 衡阳, 南华大学附属第一医院肿瘤放疗科 | | | 王贞 | 421001 衡阳, 南华大学医学院应用解剖与生殖医学研究所 | | | 曹文宇 | 421001 衡阳, 南华大学医学院应用解剖与生殖医学研究所 | | | 徐杨 | 421001 衡阳, 南华大学医学院生理教研室 | | | 刘政海 | 421001 衡阳, 南华大学医学院应用解剖与生殖医学研究所 | | | 何洁 | 421001 衡阳, 南华大学医学院病理教研室 | | | 陈熙 | 421001 衡阳, 南华大学医学院应用解剖与生殖医学研究所 | | | 何淑雅 | 421001 衡阳, 南华大学公共卫生学院 | | | 艾小红 | 421001 衡阳, 南华大学附属第一医院肿瘤放疗科 | | | 万炜 | 421001 衡阳, 南华大学医学院应用解剖与生殖医学研究所 | david-wan@163.com |
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| 中文摘要: |
| 目的 研究丰富环境对辐射诱导小鼠认知功能障碍的保护作用及其可能机制。方法 将45只2月龄雌性昆明小鼠采用随机数表法分为对照组、照射组和照射丰富环境组，每组15只。照射组和照射丰富环境组予以单次4 Gy全身137Cs γ射线照射，照射丰富环境组辐射后连续35 d给予丰富环境刺激。新旧事物识别实验检测小鼠认知功能；免疫组织化学方法检测小鼠海马区小胶质细胞标记物IBA-1的表达；Western blot方法检测小胶质细胞激活标记物CD68及突触囊泡素（SYP）的表达。结果 与对照组相比，照射组小鼠在新旧事物识别实验中新事物分辨率降低，海马区IBA-1阳性细胞数目增加，CD68蛋白表达升高，SYP蛋白表达降低（t=3.66、6.83、5.79、6.84，P<0.05）。与照射组相比，照射丰富环境组小鼠新事物分辨率升高，海马区IBA-1阳性细胞数目减少，CD68蛋白表达降低，SYP蛋白表达增加（t=3.56、7.69、4.59、4.06，P<0.05）。结论 4 Gy单次全身137Cs γ射线照射可构建放射性认知功能障碍模型，丰富环境可改善模型小鼠认知功能，其机制可能与抑制海马区小胶质细胞激活以及减少神经元突触丢失有关。 |
| 英文摘要: |
| Objective To investigate the protective effect and mechanism of environmental enrichment (EE) on radiation induced cognitive dysfunction in mice. Methods A total of 45 female Kunming mice (2-month old) were randomly divided into control group, irradiation group and irradiation plus EE group with 15 in each group. Irradiation group and irradiation plus EE group were treated with a single dose of 4 Gy whole body irradiation, irradiation plus EE group were housed in EE condition for 35 d after irradiation. The object recognition task was used to evaluate the cognitive function of mice. The expression of microglial marker IBA-1 in hippocampus was determined by immunohistochemical staining. The expressions of CD68 and synaptophysin (SYP) proteins in hippocampus were assayed by Western blot. Results Compared with control group, the irradiation group had a low discrimination ratio in object recognition task and had a remarkable low level of SYP expression in hippocampus (t=3.66, 6.84, P<0.05). In addition, radiation activated microglia in hippocampus by increasing the number of IBA-1-positive cells and enhancing the expression of CD68 (t=6.83, 5.79, P<0.05). Compared with irradiation group, irradiation plus EE group increased the discrimination ratio and the expression of SYP in hippocampus (t=3.56, 4.06, P<0.05), while the number of IBA-1-positive cells and the expression of CD68 were significantly reduced (t=7.69, 4.59, P<0.05). Conclusions A single dose of 4 Gy whole body irradiation leads to cognitive dysfunction in mice, while EE could effectively improve the animals' cognitive behavior possibly by inhibiting microglial activation and preventing synapse loss in hippocampus. |
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