| 王津晗,路倩颖,纪凯华,杜利清,王彦,徐畅,刘强.基于转录组测序的放射性肠损伤基因动态变化[J].中华放射医学与防护杂志,2018,38(2):81-86 |
| 基于转录组测序的放射性肠损伤基因动态变化 |
| The dynamic changes of gene expressions in radiation-injured intestine based on RNA-sequence analysis |
| 投稿时间：2017-09-25 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.02.001 |
| 中文关键词: 转录组测序 放射性肠损伤 差异表达基因 信号通路 基因相互作用网络 |
| 英文关键词:RNA-seq Radiation-induced intestinal injury Differential expression genes Signaling pathways Gene interaction network |
| 基金项目:国家自然科学基金（31670859） |
|
| 摘要点击次数: 3790 |
| 全文下载次数: 1928 |
| 中文摘要: |
| 目的 探讨致死剂量的电离辐射对小鼠空肠组织转录组动态变化。方法 小鼠经14 Gy 137Cs γ射线腹部照射后，分别于6 h，3.5和5 d提取各组小鼠空肠总RNA进行转录组测序。表达变化倍数在2倍以上即视为显著差异，对表达差异的基因进行IPA、Funrich、GO和KEGG软件分析。结果 小鼠在腹部照射后6 h和3.5 d共同激活了p53信号通路。在照射后第3.5天的差异基因中，基因相互作用网络分析结果表明，Lck、Cdk1和Fyn可能起关键作用，通路分析表明，上调了DNA损伤修复信号通路，下调了细胞黏附分子、黏着斑和IgA分泌途径信号通路。结论 p53信号通路以及Lck、Cdk1和Fyn等基因在放射性肠损伤中可能起关键作用。 |
| 英文摘要: |
| Objective To investigate the dynamic changes of gene expressions in mouse jejunum after lethal dose abdomen irradiation(ABI). Methods RNA was extracted from mouse jejunum at 0 and 6 h, 3.5 and 5 d after 14 Gy 137Cs γ-ray ABI and then subjected to RNA-sequence analysis. Gene with expressions changed more than 2-fold of control were identified as differentially expressed ones. The selected genes were subsequently analyzed using IPA, Funrich, GO and KEGG software. Results Gene analysis of mouse jejunum samples showed that radiation activated p53 pathway at 6 h and 3.5 d after ABI. Interaction network analysis of genes suggested that Lck, Cdk1 and Fyn, genes could play an important role in jejunum damage at 3.5 d after ABI. The gene expression profiles demonstrated that ABI up-regulated DNA damage repair pathways and down-regulated cell adhesion molecules, focal adhesion and IgA production pathways. Conclusions The p53 signaling pathway and some key genes such as Lck, Cdk1, and Fyn may contribute to the radiation-induced intestinal injury. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|