| 刘明珠,樊锐太,顾浩,等.吴茱萸碱对子宫内膜癌细胞放射敏感性的影响[J].中华放射医学与防护杂志,2018,38(1):6-11.Liu Mingzhu,Fan Ruitai,Gu Hao,et al.Effect of evodiamine on the proliferation and radiosensitivity of endometrial carcinoma cells[J].Chin J Radiol Med Prot,2018,38(1):6-11 |
| 吴茱萸碱对子宫内膜癌细胞放射敏感性的影响 |
| Effect of evodiamine on the proliferation and radiosensitivity of endometrial carcinoma cells |
| 投稿时间：2017-08-26 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.01.002 |
| 中文关键词: 吴茱萸碱 子宫内膜癌 放射敏感性 增殖 |
| 英文关键词:Evodiamine Endometrial carcinoma Radiation sensitivity Proliferation |
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| 中文摘要: |
| 目的 探讨吴茱萸碱对子宫内膜癌细胞放射敏感性及增殖、凋亡的影响。方法 用不同浓度的吴茱萸碱作用于人子宫内膜癌细胞Ishikawa、HEC-1A、AN3CA，四甲基偶氮唑盐（MTT）检测细胞增殖情况，计算半数抑制浓度，筛选出受到增殖抑制作用最大的癌细胞。分别用半数抑制浓度的吴茱萸碱、8 Gy照射处理细胞，同时用吴茱萸碱联合射线照射细胞，流式细胞术检测细胞凋亡情况，Western blot检测细胞中活化的含半胱氨酸的天冬氨酸蛋白水解酶3（Cleaved Caspase-3）、p38、磷酸化的p38（p-p38）水平，试剂盒检测细胞中活性氧（ROS）水平，细胞克隆实验检测细胞放射敏感性。结果 1、2、4、8 μmol/L的吴茱萸碱能够抑制人子宫内膜癌细胞Ishikawa、HEC-1A、AN3CA增殖，半数抑制浓度IC50分别为（8.32±0.95）、（3.98±0.84）、（4.78±0.64）μmol/L，吴茱萸碱对人子宫内膜癌细胞HEC-1A抑制作用最强，后续研究中选用4 μmol/L的吴茱萸碱作用于人子宫内膜癌细胞HEC-1A。吴茱萸碱联合放疗处理后HEC-1A细胞凋亡率由（45.54±4.25）%升高到（65.87±2.93）%（t=11.010，P<0.05）。细胞存活率由（41.84±4.18）%下降到（33.27±3.52）%（t=7.484，P<0.05）。计算吴茱萸碱作用后的HEC-1A细胞放疗增敏比为1.628。吴茱萸碱联合放疗处理后较单纯放疗处理后细胞中ROS水平及Cleaved Caspase-3、p-p38水平升高。结论 吴茱萸碱能够抑制子宫内膜癌细胞增殖，促进子宫内膜癌细胞凋亡。吴茱萸碱能够增强子宫内膜癌细胞放射敏感性，作用机制可能与细胞内ROS水平及细胞中p38信号通路有关。 |
| 英文摘要: |
| Objective To investigate the effect of evodiamine on the proliferation and sensitivity of endometrial cancer cells to irradiation. Methods After administration of evodiamine, cell proliferations of human endometrial carcinoma cell lines of Ishikawa, HEC-1A, AN3CA were detected by MTT and the half maximal inhibitory concentration (IC50) of drug was calculated. The cell lines most sensitive to drug were screened for further experiment and administered with evodiamine (IC50) and 8 Gy irradiation. Then, cell apoptosis was detected by flow cytometry, the levels of Cleaved Caspase-3, p38 and p-p38 were measured by Western blot, and the level of intracellular ROS was detected by a ROS kit. Cell clone survival was also detected to evaluate cell radiosensitivity. Results 1, 2, 4, 6 and 8 μmol/L evodiamine could inhibit the proliferation of the cell line of Ishikawa, HEC-1A, and AN3CA with IC50 of(8.32±0.95),(3.98±0.84) and (4.78±0.64)μmol/L, respectively. Compared with radiation alone, after radiation in combination with 4 μmol/L evodiamine, the apoptosis rate of HEC-1A cells was increased from (45.54±4.25)% to (65.87±2.93)% (t=11.010,P<0.05) and cell viability decreased from (41.84±4.18)% to (33.27±3.52)% (t=7.484,P<0.05), and the levels of ROS, Cleaved Caspase-3 and p-p38 were also enhanced. In addition, the sensitivity ratio of evodiamine for HEC-1A cells was calculated to be 1.628. Conclusions Evodiamine could inhibit the proliferation, promote apoptosis and enhance the radiosensitivity of endometrial carcinoma cells, in which the intracellular ROS and p38 signaling pathway may be involved. |
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