| 苏晓雨,徐慧琴,汪会,张丹,谯凤,余文静.马蔺子素放射增敏作用对MDA-MB231细胞Warburg效应的影响[J].中华放射医学与防护杂志,2018,38(1):1-5 |
| 马蔺子素放射增敏作用对MDA-MB231细胞Warburg效应的影响 |
| Radiosensitization of irisquinone on the Warburg effect of MDA-MB231 cells |
| 投稿时间：2017-06-15 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.01.001 |
| 中文关键词: 马蔺子素 放射增敏 Warburg效应 己糖激酶-Ⅱ |
| 英文关键词:Irisquinone Radiosensitization Warburg effect HKⅡ |
| 基金项目:国家自然科学基金（81371587） |
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| 中文摘要: |
| 目的 研究马蔺子素放射增敏作用在人乳腺癌MDA-MB231细胞照射过程中对Warburg效应的影响。方法 取指数生长期的人乳腺癌MDA-MB231细胞，分为空白对照组、马蔺子素组、单纯照射组、马蔺子素+照射组、阴性对照组及转染己糖激酶-Ⅱ（HKⅡ） siRNA的实验组。克隆形成实验检测MDA-MB231细胞的存活分数；单击多靶模型拟合细胞存活曲线并计算放射增敏比（SER）；用流式细胞仪检测各组细胞凋亡情况；通过qRT-PCR和Western blot分别检测各组细胞HKⅡmRNA和蛋白相对表达水平。结果 马蔺子素+照射组的D0、Dq及SF2较单纯照射组均有所下降，放射增敏比（SER）为1.52。马蔺子素+照射组凋亡率高于空白对照组、马蔺子素组、单纯照射组、转染HKⅡsiRNA组，差异有统计学意义（t=13.29、12.09、5.90、3.83，P < 0.05）。马蔺子素+照射组与空白对照组、马蔺子素组、单纯照射组相比，HKⅡmRNA的相对表达水平明显降低，差异有统计学意义（t=9.14、10.48、3.40，P < 0.05），HKⅡ蛋白的相对表达水平也明显降低，差异有统计学意义（t=13.39、16.08、5.81，P < 0.05）。结论 马蔺子素放射增敏作用能通过下调HKⅡ基因的表达，从而抑制MDA-MB231细胞的Warburg效应。 |
| 英文摘要: |
| Objective To study the radiosensitization of irisquinone on the Warburg effect of MDA-MB231 cells. Methods MDA-MB231 cells in the exponential growth phase were divided into 6 groups:control group, irisquinone group, radiation group, irisquinone plus radiation group(Irisquinone+RA), negative control group, and experimental group (siRNA). Colony formation assay was used to measure cell survival fraction of MDA-MB231 cells. Single-hit multi-target model was used to fit the survival curve and calculate the sensitive enhancement ratio (SER). Flow cytometry was used to measure cell apoptosis. The relative expression levels of HK Ⅱ mRNA and protein in each group were detected by qRT-PCR and Western blot respectively. Results The values of D0, Dq and SF2 in the irisquinone plus radiation group were obviously lower than those in the radiation group. The SER of irisquinone was 1.52. Compared with the other groups, the cell apoptosis rate was increased (t=13.29,12.09,5.90,3.83, P < 0.05),while the relative expression levels of HK Ⅱ mRNA (t=9.14, 10.48, 3.40,P < 0.05) and protein (t=13.39, 16.08, 5.81,P < 0.05) were decreased in the irisquinone plus radiation group significantly. Conclusions Radiosensitization function of irisquinone inhibits the Warburg effect of MDA-MB231 cells by down-regulating the expression of HKⅡ. |
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