| 杨颖,李志鹏,贾春丽,等.薏苡仁注射液提高人肝癌细胞Bel-7402放射敏感性的机制研究[J].中华放射医学与防护杂志,2017,37(12):885-890.Yang Ying,Li Zhipeng,Jia Chunli,et al.Mechanism of Coix seed injection enhances the radiosensitivity in hepatocellular carcinoma cell Bel-7402[J].Chin J Radiol Med Prot,2017,37(12):885-890 |
| 薏苡仁注射液提高人肝癌细胞Bel-7402放射敏感性的机制研究 |
| Mechanism of Coix seed injection enhances the radiosensitivity in hepatocellular carcinoma cell Bel-7402 |
| 投稿时间：2017-05-27 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.12.001 |
| 中文关键词: 放射敏感性 薏苡仁注射液 X射线 肝癌细胞 |
| 英文关键词:Radiosensitivity Coix seed injection X-rays Hepatoma cell |
| 基金项目:国家自然科学基金（81560388） |
| 作者 | 单位 | E-mail | | 杨颖 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 李志鹏 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 贾春丽 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 刘强 | 830011 乌鲁木齐, 新疆医科大学第一附属医院泌尿中心 | | | 杨志芳 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 张瑞丽 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 毛睿 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 张华 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 艾斯克尔·吐拉洪 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | | | 包永星 | 830011 乌鲁木齐, 新疆医科大学第一附属医院肿瘤中心 | baoyx@vip.sina.com |
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| 中文摘要: |
| 目的 观察薏苡仁注射液对人肝癌细胞Bel-7402活性和放射敏感性的影响。方法 将Bel-7402细胞分为空白对照组、薏苡仁组、单纯照射组和联合处理组。采用X射线照射，同时联合薏苡仁注射液处理，采用四甲基偶氮唑盐（MTT）比色法检测细胞增殖活性的变化，流式细胞术观察细胞凋亡率的变化，应用RT-PCR和Western blot法检测肝癌细胞中Bax、Bcl-2 mRNA和蛋白的表达变化。采用细胞克隆形成法观察存活细胞数，单击多靶模型拟合剂量存活曲线，计算细胞平均致死剂量（D0）值。结果 薏苡仁注射液和X射线对肝癌细胞的生长均有明显抑制作用，其中联合处理组的细胞抑制率明显高于薏苡仁组和单纯照射组（8 Gy），差异有统计学意义（t=17.03、11.26，P<0.05）；与薏苡仁组和单纯照射组相比，联合处理组的细胞凋亡率明显增加（t=24.80、20.19，P<0.05）。薏苡仁组、单纯照射组及联合处理组Bax的mRNA和蛋白水平依次升高（F=437.92、67.91，P<0.05），而Bcl-2的mRNA和蛋白水平逐渐降低（F=31.18、48.50，P<0.05）。单纯照射和联合处理组D0值分别是4.27和3.34，放射增敏比为1.27。结论 薏苡仁注射液可能通过凋亡相关因子Bax和Bcl-2抑制肝癌细胞生长，诱导凋亡，具有放射增敏作用。 |
| 英文摘要: |
| Objective To observe the effects of Coix seed injection on the cell viability and radiosensitivity of human hepatoma cell line Bel-7402. Methods Bel-7402 cells were irradiated by X-rays, or treated with Coix seed injection, or treated with both of them. The cells proliferation and apoptosis were detected by MTT and by flow cytometry respectively. Cell cloning was used to observe the number of viable cells and to draw the cell survival curve. The mRNA and protein level of Bax, Bcl-2 were detected by RT-PCR and Western blot respectively. Results It was found that the Coix seed injection group(12 μmol/L) and X-ray group(8 Gy)had a significant inhibitory effect on the growth of hepatocellular carcinoma cells(t=17.03, 11.26, P<0.05).And compared with Coix group and irradiation group, the combined treatment group showed higher inhibition rate(t=24.80, 20.19, P<0.05). The mRNA and protein levels of Bax were gradually elevated(F=437.92, 67.91,P<0.05), while the expressions of Bcl-2 reflected a decreased trend (F=31.18, 48.50,P<0.05). The D0 values of pure irradiation group and combined treatment group were 4.27 and 3.34, respectively, and the sensitization enhancement ratio was 1.27. Conclusions The Coix seed injection inhibit cell growth and induce apoptosis, as well as increase radiative sensitization may via the apoptosis related factors Bax and Bcl-2 in hepatocellular carcinoma. |
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