| 孙菲,孙志强,汪建林,等.阿帕替尼对食管癌Kyse-150细胞放射敏感性影响的实验研究[J].中华放射医学与防护杂志,2017,37(11):805-809,831.Sun Fei,Sun Zhiqiang,Wang Jianlin,et al.Radiosensitizing effect of apatinib on esophageal cancer cell line Kyse-150[J].Chin J Radiol Med Prot,2017,37(11):805-809,831 |
| 阿帕替尼对食管癌Kyse-150细胞放射敏感性影响的实验研究 |
| Radiosensitizing effect of apatinib on esophageal cancer cell line Kyse-150 |
| 投稿时间：2017-06-25 修订日期：2017-06-25 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.11.001 |
| 中文关键词: 食管癌 放射敏感性 阿帕替尼 细胞凋亡 细胞周期 |
| 英文关键词:Esophageal cancer Radiosensitivity Apatinib Apoptosis Cell cycle |
| 基金项目:国家自然科学基金（11705095） |
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| 中文摘要: |
| 目的 观察阿帕替尼对食管癌Kyse-150细胞放射敏感性的影响，并初步探讨其作用机制。方法 将食管癌Kyse-150细胞分为空白对照组、阿帕替尼组、单纯照射组、阿帕替尼联合照射组。CCK-8法检测不同浓度阿帕替尼（0、5、10、20、30、40 μmol/L）对细胞增殖的影响；克隆形成法检测不同浓度阿帕替尼（0、10、20、40 μmol/L）对细胞放射敏感性的影响；流式细胞术分析阿帕替尼（20 μmol/L）联合射线（4 Gy）对细胞周期和细胞凋亡的影响。结果 阿帕替尼能抑制Kyse-150细胞的增殖，且呈剂量-时间依赖性（r=0.89~0.96，P<0.05）；随着阿帕替尼浓度的增加，Kyse-150细胞的放射生物学参数D0、Dq、SF2值逐渐减小，而放射增敏比SERD0值逐渐增加。阿帕替尼联合照射组分别与空白对照组、单纯照射组及阿帕替尼组比较，细胞凋亡率均显著增加，差异具有统计学意义（t=12.36、5.99、15.47，P<0.05）。与空白对照组比较，阿帕替尼组、单纯照射组及阿帕替尼联合照射组G2/M期比例均增高，差异均具有统计学意义（t=8.81、39.69、20.61，P<0.05）。阿帕替尼组、阿帕替尼联合照射组分别与空白对照组及单纯照射组相比，S期比例均增高，差异均具有统计学意义（t=6.06、3.82、8.81、6.24，P<0.05）。而阿帕替尼组与阿帕替尼联合照射组相比，S期比例差异无统计学意义（P> 0.05）。结论 阿帕替尼能增加食管癌Kyse-150细胞的放射敏感性，其机制可能与抑制细胞增殖、促进细胞凋亡及诱导细胞周期再分布有关。 |
| 英文摘要: |
| Objective To evaluate the radiosensitization effect of apatinib on esophageal cancer cell line Kyse-150, and to investigate the underlying mechanism. Methods Cells were divided into four groups:control group, apatinib treatment group, X-ray radiation group, and the combination group treated with X-rays plus apatinib. The effect of apatinib with different concentrations on the cell proliferative and radiosensitivity were evaluated by CCK-8 kit and colony formation assay. Flow cytometry method was adopted to detect the effect of apatinib on cell cycle progress and apoptosis induction. Results Apatinib inhibited the proliferation of Kyse-150 cells in time-and dose-dependent manners (r=0.89-0.96, P<0.05). With the increase of apatinib concentration, D0, Dq and SF2 value of Kyse-150 cells decreased and SERD0 value increased. Compared with control group, apatinib alone group, and radiation alone group, the cell apoptosis rate significantly increased in the combination group (t=12.36, 5.99, 15.47,P<0.05). Compared with control group, the percentages of cells in G2/M phase were all significantly increased in apatinib group, radiation group and combination group (t=8.81, 39.69, 20.61,P<0.05). Compared with radiation alone group and control group, the percentage of cells in S phase significantly increased in apatinib alone group and combination group(t=6.06, 3.82,8.81,6.24,P<0.05). ConclusionsApatinib can increase radiosensitivity of esophageal cancer cell line Kyse-150 possibly by inhibiting cell proliferation, inducing cell apoptosis and causing redistribution of cell cycle. |
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