| 刘明珠,樊锐太,顾浩,等.小檗碱增强宫颈癌细胞放射敏感性的研究[J].中华放射医学与防护杂志,2017,37(8):581-586.Liu Mingzhu,Fan Ruitai,Gu Hao,et al.Effect of berberine on radiosensitivity of cervical cancer cells[J].Chin J Radiol Med Prot,2017,37(8):581-586 |
| 小檗碱增强宫颈癌细胞放射敏感性的研究 |
| Effect of berberine on radiosensitivity of cervical cancer cells |
| 投稿时间：2017-01-20 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.08.004 |
| 中文关键词: 宫颈癌 小檗碱 放射敏感性 信号通路 细胞凋亡 |
| 英文关键词:Cervical cancer Berberine Radiosensitivity Signaling pathway Cell apoptosis |
| 基金项目:福建省中青年骨干项目（2013-ZQN-ZD-8）；福建省自然科学基金（2016J01437） |
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| 中文摘要: |
| 目的 探讨小檗碱对宫颈癌细胞凋亡及放射敏感性的影响。方法 用5、10、15、20 μmol/L的小檗碱作用于宫颈癌细胞Siha、HeLa、Caski记为给药组，同时以只加入二甲基亚砜（DMSO）的细胞为对照组，CCK-8法检测48 h细胞存活率，计算小檗碱半数抑制浓度。用半数抑制浓度的小檗碱作用于宫颈癌Siha细胞48 h，流式细胞术检测细胞凋亡率和细胞周期，Western blot检测细胞中活化的含半胱氨酸的天冬氨酸蛋白水解酶3（Cleaved Caspase-3）、细胞周期素B1（Cyclin Bl）、细胞周期蛋白依赖性激酶1（CDK1）、信号转导和转录因子（STAT）、磷酸化的STAT3（p-STAT3）表达水平。含有半数抑制浓度的小檗碱培养液培养宫颈癌Siha细胞24 h后，用0、2、4、6、8 Gy X射线照射细胞，细胞克隆形成实验检测放射敏感性。结果 小檗碱能够抑制宫颈癌细胞的生长，抑制作用从大到小为：Siha、Caski、HeLa，半数抑制浓度依次为（16.84±3.52）、（23.54±8.67）、（21.86±6.35）μmol/L。17 μmol/L的小檗碱能够促进宫颈癌细胞Siha凋亡（t=56.847，P<0.01），将细胞周期阻滞在G2/M期（t=47.251，P<0.01），并抑制宫颈癌细胞中Cyclin B1、CDK1、p-STAT3表达，促进Cleaved Caspase-3表达，对宫颈癌细胞中STAT3表达没有影响。17 μmol/L的小檗碱能够降低宫颈癌细胞的存活分数，放射增敏比为1.550。结论 小檗碱能够抑制宫颈癌细胞增殖，促进凋亡，阻滞细胞周期，增加宫颈癌细胞的放射敏感性。 |
| 英文摘要: |
| Objective To investigate the effect of berberine on the radiosensitivity of cervical cancer cells. Methods 5, 10, 15, 20 μmol/L of berberine were used to treat cervical cancer cell lines of Siha, HeLa, Caski. DMSO was applied as control of drug treatment. Cell proliferation was detected by the CCK-8 method, and then the half inhibitory concentration of berberine was calculated. Cell apoptosis and cell cycle distribution were detected by flow cytometry. Protein expressions of Cleaved Caspase-3, Cyclin B1, CDK1, STAT3 and p-STAT3 were detected by Western blot. Cervical cancer cells of Siha were treated by berberine with a half inhibitory concentration for 24 h and then irradiated with 0, 2, 4, 6, 8 Gy of X-rays. Cell clone assay was used to detect cell survival. Results Berberine could inhibit the growth of cervical cancer cells with a half inhibition concentration of(16.84±3.52),(23.54±8.67),(21.86±6.35)μmol/L for Siha, Caski, and HeLa cells, respectively. The berberine at 17 μmol/L could induce apoptosis(t=56.847,P<0.01)and G2/M phase arrest(t=47.251,P<0.01)in Siha cells, which also inhibited the expressions of Cyclin B1, CDK1 and p-STAT3 and promoted the expression of cleaved Caspase-3, but did not influence the expression of STAT3 in cervical cancer cells. Treatment of cells with 17 μmol/L berberine increased the radiosensitivity of cervical cancer cells with a sensitivity enhancement ratio of 1.55. Conclusions Berberine can inhibit cell proliferation, promote apoptosis, block cell cycle, and increase radiosensitivity of cervical cancer cells. |
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