| 韩晓丹,薛晓蕾,张俊伶,樊赛军.富氢水减轻造血干祖细胞的辐射损伤[J].中华放射医学与防护杂志,2017,37(5):327-331 |
| 富氢水减轻造血干祖细胞的辐射损伤 |
| Hydrogen-rich water alleviates radiation-induced injury to hematopoietic stem and progenitor cells |
| 投稿时间：2017-01-09 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.05.002 |
| 中文关键词: 富氢水 电离辐射 造血干祖细胞 活性氧 |
| 英文关键词:Hydrogen-rich water Ionizing radiation Hematopoietic stem and progenitor cells Reactive oxygen species |
| 基金项目:国家自然科学基金（81402633，81572969）；科技部科研院所技术发展研究专项（2014EG150134）；天津科技支撑计划项目（14ZCZDSY00001）；天津市自然科学基金青年项目（16JCQNJC13600） |
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| 中文摘要: |
| 目的 探讨富氢水对辐射诱导的造血干祖细胞（HSPCs）损伤的保护作用。方法 32只C57BL/6小鼠根据体重分层随机区组法分为健康对照组、富氢水组、照射组、照射+富氢水组，共4组，每组8只。富氢水组和照射+富氢水组小鼠于照射前5 min至照后7 d，每天灌胃给予0.5 ml富氢水，其余小鼠每天灌胃给予0.5 ml蒸馏水，照射组和照射+富氢水组小鼠接受2 Gy的137Cs γ射线全身照射。照后15 d取小鼠骨髓，检测骨髓中HSPCs比例、骨髓细胞的克隆形成和移植重建能力、骨髓中LSK细胞的活性氧（ROS）水平和细胞凋亡情况。结果 与照射组相比，照射+富氢水组小鼠骨髓中造血祖细胞和LSK细胞比例升高（t=-4.935、-7.898，P<0.05），骨髓细胞形成克隆的数目增加（t=5.488，P<0.05），竞争性骨髓移植后受体小鼠的供体嵌合率升高（t=-12.769，P<0.05），骨髓中LSK细胞的ROS水平和细胞凋亡比例降低（t=4.380、3.954，P<0.05）。结论 富氢水对2 Gy电离辐射诱导的HSPCs损伤具有一定的保护作用。 |
| 英文摘要: |
| Objective To investigate the protective effect of hydrogen-rich water (HRW) on radiation-induced hematopoietic stem and progenitor cells (HSPCs) injury.Methods Totally 32 C57BL/6 mice were randomly divided into four groups with 8 mice in each group, including control, HRW, radiation and radiation + HRW. Mice in HRW and radiation + HRW groups received 0.5 ml hydrogen-rich water per day by intragastric administration 5 min before irradiation until 7 d post-irradiation. Mice in other groups received 0.5 ml distilled water. Mice in radiation and radiation + HRW group were irradiated with 2 Gy of total body irradiation. Bone marrow cells were isolated at 15 d post-irradiation, and LSK cells were examined for the percentage of hematopoietic stem and progenitor cells, the ability of colony formation and reconstitution, reactive oxygen species (ROS) levels and cell apoptosis. Results Compared with radiation group, the percentages of hematopoietic progenitor cells and LSK cells, colony number of bone marrow cells were significantly increased in radiation + HRW group (t=-4.935,-7.898,5.488,P<0.05). An elevation of donor chimerism was also found in recipient mice administered HRW after competitive bone marrow transplantation (t=-12.769,P<0.05). Compared with radiation group, the ROS levels and cell apoptosis in LSK cells were significantly decreased (t=4.380,3.954, P<0.05). Conclusions Hydrogen-rich water exhibited a protective effect on radiation-induced HSPCs injury. |
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