| 孙超南,乔俏,李光,韩楚阳,韩凝,张妙.内质网应激通路抑制剂Salubrinal增强人头颈部鳞癌细胞的放射敏感性[J].中华放射医学与防护杂志,2017,37(3):177-181 |
| 内质网应激通路抑制剂Salubrinal增强人头颈部鳞癌细胞的放射敏感性 |
| Endoplasmic reticulum stress inhibitor salubrinal enhanced radiosensitivity of head and neck carcinoma cells |
| 投稿时间：2016-10-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.03.003 |
| 中文关键词: 头颈部鳞癌 放射敏感性 内质网应激通路抑制剂 葡萄糖调节蛋白78 |
| 英文关键词:Head and neck squamous carcinoma Radiosensitivity Endoplasmic reticulum stress pathway inhibitor Glucose regulated proteins 78 |
| 基金项目:国家自然科学基金（81402521） |
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| 中文摘要: |
| 目的 观察内质网应激通路抑制剂Salubrinal增强人头颈部鳞癌细胞放射敏感性的作用。方法 采用Western blot法检测人头颈部鳞癌细胞系（KB、Fadu、Detroit 562）射线照射后不同时间点（0、20 min、1 h、3 h、6 h、12 h、24 h和48 h）内质网应激通路核心蛋白-葡萄糖调节蛋白78（GRP78）的表达情况；对3种细胞系设立空白对照组和ERS通路抑制剂组（sal组），同样方法检测GRP78的表达；集落克隆形成法检测3种细胞系不同剂量照射（0、2、4和6 Gy）对10 mmol/L Salburinal处理后不同时间（12、24、36 h）细胞存活分数的影响。结果 Western blot结果显示，照射后20 min~1 h，3种头颈部鳞癌细胞GRP78蛋白表达增加，照射后3 h达到高峰，与0 min组比较，差异有统计学意义（t=12.72、13.37、18.31，P<0.05）。采用Salubrinal处理细胞后，sal组与空白对照组比较，GRP78的表达下调，差异有统计学意义（t=14.25、5.34、3.12，P<0.05）。集落克隆形成实验结果提示，Salubrinal增强射线抑制细胞增殖，3种细胞加药12 h的放射增敏比分别为1.16、1.05和1.06。结论 内质网应激通路抑制剂Salubrinal增强人头颈部鳞癌细胞放射敏感性。 |
| 英文摘要: |
| Objective To explore the effect of salubrinal (sal, an endoplasmic reticulum stress inhibitor) on radiosensitivity of human head and neck squamous carcinoma cells (HNSCC). Methods Cells were divided into two groups of sal treatment and its control. For drug treatment group, cells were treated with 10 mmol/L sal for different time (12, 24, 36 h) and then irradiated. The levels of a core protein GRP78 of endoplasmic reticulum stress (ERS) in HNSCC(KB, Fadu, and Detroit 562 cells)were analyzed by Western blot assay at different time (0, 20 min,1 h, 3 h, 6 h, 12 h, 24 h and 48 h) after irradiation. Cell survival was measured with colony formation assay. Results Western blot assay revealed that the protein levels of GRP78 in three kinds of HNSCC significantly increased from 20 min to 1 h and peaked at 3 h after radiation (t=12.72, 13.37, 18.31, P<0.05). Compared with the control group, treatment of cells with sal decreased GRP78 protein levels (t=14.25, 5.34, 3.12, P<0.05) in three cell lines and also significantly enhanced radiation damage and reduced cell viability. The sensitization enhancement ratios (SER) of sal in three cell lines were 1.16, 1.05 and 1.06, respectively. Conclusions Rradiosensitivity of HNSCC could be effectively enhanced by sal treatment. |
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