中华放射医学与防护杂志

宋淑军,司少艳,刘俊丽,左晓勇,王莹,郭燕川,单改仙,王宗烨.胶原蛋白肽对X射线照射小鼠免疫功能的影响[J].中华放射医学与防护杂志,2016,36(11):811-814

胶原蛋白肽对X射线照射小鼠免疫功能的影响

The effect of the collagen peptides on the immune function of X-ray irradiated mice

投稿时间：2016-07-12

DOI：10.3760/cma.j.issn.0254-5098.2016.11.003

英文关键词:Collagen peptides Immune function X-rays

基金项目:

作者	单位	E-mail
宋淑军	100101 北京, 解放军第306医院特种医学实验研究中心
司少艳	100101 北京, 解放军第306医院特种医学实验研究中心
刘俊丽	100101 北京, 解放军第306医院特种医学实验研究中心
左晓勇	100101 北京, 解放军第306医院放疗中心
王莹	100101 北京, 解放军第306医院放疗中心
郭燕川	100190 北京, 中国科学院理化技术研究所光化学转换与功能材料重点实验室
单改仙	100101 北京, 解放军第306医院放疗中心
王宗烨	100101 北京, 解放军第306医院放疗中心	wangzye@163.com

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中文摘要:

目的了解胶原蛋白肽（CP）对X射线照射小鼠免疫功能的调节作用。方法 ICR小鼠按体重分层随机分为5组，分别为健康对照组、2 Gy、5 Gy、2 Gy+CP 600 mg·kg^-1·d^-1组（2 Gy CP）和5 Gy+CP 600 mg·kg^-1·d^-1组（5 Gy CP）。6 MV X射线单次全身照射诱导免疫抑制小鼠模型，剂量率为6 Gy/min。2 Gy CP和5 Gy CP组的小鼠连续7 d腹腔注射给予胶原蛋白肽600 mg/kg。测量小鼠体质量、胸腺和脾脏质量，计算胸腺和脾脏指数；检测刀豆蛋白A（ConA）诱导的脾脏T淋巴细胞增殖能力及脾脏T淋巴细胞分泌的白介素-2（IL-2）的水平。结果与健康对照组相比，X射线照射小鼠的胸腺指数、脾脏指数、脾脏T 淋巴细胞增殖能力及IL-2浓度显著降低（F=76.857、181.870、63.133、8.499，P<0.05）。腹腔注射胶原蛋白肽后，与同等剂量单纯照射小鼠比较，上述指标均有所恢复。结论腹腔注射胶原蛋白肽能够增强X射线照射小鼠的免疫功能。

英文摘要:

Objective To study the effects of collagen peptides(CP) on the immune functions of X-ray irradiated mice. Methods ICE mice were randomly divided into five groups according to body weight:control, 2 Gy, 5 Gy, 2 Gy CP (2 Gy X-ray irradiation & administrated with collagen peptides 600 mg·kg^-1·d^-1) and 5 Gy CP (5 Gy X-ray irradiation & administrated with collagen peptides 600 mg·kg^-1·d^-1) groups. Immunosuppressant mouse model was established by iradiating ICE mice with 6 MV of X-rays generated by a linear accelerator, where the whole body of the mice was irradiated with a single dose of 2 Gy or 5 Gy at dose rate of 6 Gy/min. And the mice in groups of 2 and 5 Gy CP were injected intraperitoneally with collagen peptides (600 mg·kg^-1·d^-1) for 7 days after irradiation. On the day 8, the mice were decapitated. The body weights, thymus and spleen indexes, IL-2 level and proliferations of spleen T lymphocytes induced by ConA were detected. Results Compared with the control group, the thymus and spleen indexes, the level of IL-2 and proliferations of spleen T lymphocytes were significantly decreased(F=76.857, 181.870, 63.133, 8.499, P<0.05). These parameters were restored to some extent in mice injected with collagen peptides when compared with the mice irradiated with same dose of X-rays. Conclusion Peritoneal injection of collagen peptides can enhance the immune functions in X-ray irradiated mice.

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