| 彭啟亮,林宇鑫,袁徐烨,朱雅群.MicroRNA及其靶基因在预测直肠癌放化疗疗效中的作用[J].中华放射医学与防护杂志,2016,36(10):743-748,752 |
| MicroRNA及其靶基因在预测直肠癌放化疗疗效中的作用 |
| Roles of microRNAs and their target genes in predicting chemoradiotherapy efficacy of rectal cancer |
| 投稿时间：2016-05-15 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.10.005 |
| 中文关键词: 直肠癌 放化疗 microRNA 靶基因 生物信息学 |
| 英文关键词:Rectal cancer Chemoradiotherapy MicroRNA Target gene Bioinformatics |
| 基金项目: |
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| 中文摘要: |
| 目的 MicroRNA及其靶基因参与直肠癌放化疗疗效应答，本研究旨在筛选直肠癌放化疗疗效相关的microRNA及其靶基因，推动直肠癌放化疗疗效的基础研究。方法 基于PubMed数据库挖掘与直肠癌放化疗疗效相关的microRNA，结合microRNA-mRNA调控关系以及基因芯片表达谱数据，寻找与直肠癌放化疗疗效相关的靶基因。通过DAVID、IPA等工具对靶基因进行基因本体论和信号转导通路富集分析。结果 通过文本挖掘，共搜集到38个与直肠癌放化疗相关的microRNA，结合实验验证和计算机预测的microRNA-mRNA调控关系，共得到潜在的靶基因3 545个。其中，131个在基因芯片表达谱（GSE35452）中具有显著的差异表达现象（P<0.05）。基因本体论以及信号转导通路富集结果表明，这些基因与直肠放化疗疗效密切相关。结论 上述microRNA及其调控的差异表达基因参与了直肠癌放化疗疗效相关的多条信号通路，在一定程度上从生物机制的角度解释了直肠癌放化疗疗效的差异。同时，筛选出的microRNA及其靶基因也为预测直肠癌放化疗疗效的研究提供了理论依据。 |
| 英文摘要: |
| Objective MicroRNAs (miRNAs) play important roles in the chemoradiotherapy efficacy of rectal cancer (RC). This study aimed to screen the chemoradiotherapy-associated microRNAs and their target genes of RC through bioinformatics approaches in order to promote the fundamental study of RC chemoradiotherapy. Methods The chemoradiotherapy-associated microRNAs were manually searched through the published papers via PubMed and its target genes were identified by comprehensively analyzing these public data of microRNA-mRNA and gene expression profiles. Both gene ontology (GO) and pathway analysis of the target genes were performed by DAVID and IPA programs, respectively. Results A total of 38 microRNAs were collected from PubMed, and 3 545 putative target genes were inferred from the integrated microRNA-mRNA associations, among them, 131 were differentially expressed (DE) (P<0.05) in the selected gene expression profile (GSE35452). The GO and pathway enrichment analyses indicated that the DE genes were closely involved in the responses of chemoradiotherapy of RC. Conclusions These microRNAs and their regulated DE genes may contribute to the molecular mechanism of the differential efficacy of RC chemoradiotherapy, which may provide a theoretical reference for predicting the response of RC to chemoradiotherapy. |
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