| 明慧,高景美,房蕾,等.131I-RGD-BSA-PCL用于非小细胞肺癌荷瘤裸鼠SPECT/CT显像及抑瘤作用[J].中华放射医学与防护杂志,2016,36(9):641-647.Ming Hui,Gao Jingmei,Fang Lei,et al.Evaluation of SPECT/CT imaging and internal therapeutic effectiveness of 131I-RGD-BSA-PCL in the lung cancer mouse model[J].Chin J Radiol Med Prot,2016,36(9):641-647 |
| 131I-RGD-BSA-PCL用于非小细胞肺癌荷瘤裸鼠SPECT/CT显像及抑瘤作用 |
| Evaluation of SPECT/CT imaging and internal therapeutic effectiveness of 131I-RGD-BSA-PCL in the lung cancer mouse model |
| 投稿时间：2016-03-31 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.09.001 |
| 中文关键词: 放射性碘 精氨酸-甘氨酸-天冬氨酸(RGD) 纳米脂质体 非小细胞肺癌 |
| 英文关键词:Radioiodine Arg-Gly-Asp (RGD) Nano-liposome NSCLC |
| 基金项目:国家自然科学基金（81301244） |
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| 中文摘要: |
| 目的 探讨131I-RGD-BSA-PCL核素纳米载体对非小细胞肺癌荷瘤裸鼠模型SPECT/CT显像效果及其对肿瘤的抑制作用。方法 构建纳米脂质体131I-RGD-BSA-PCL及131I-BSA-PCL，通过荧光共聚焦显微镜观察该载体在非小细胞肺癌细胞系H460的靶向性结合及细胞摄取情况；采用氯氨T法标记核素纳米载体；流式细胞术观察核素纳米载体对肿瘤细胞的杀伤作用。构建荷瘤裸鼠模型，研究核素纳米载体在荷瘤裸鼠体内的组织分布、肿瘤体积变化及各组荷瘤裸鼠SPECT/CT断层显像。结果 给药后1和8 h，H460细胞质和细胞核对RGD-BSA-PCL、BSA-PCL两种纳米载体均有明显摄取。Na131I、131I-BSA-PCL及131I-RGD-BSA-PCL对H460细胞的早期凋亡率分别为（33.3±12.5）%、（68.4±8.0）%和（70.5±12.2）%。荷瘤裸鼠体内实验中，给药后24和72 h，肿瘤131I-RGD-BSA-PCL摄取率均高于131I-BSA-PCL（t=9.53、5.03，P<0.01）。给药后23 d，131I-RGD-BSA-PCL肿瘤体积抑制最明显（t=126.44，P<0.01）。SPECT/CT显示，给药后21 d，131I-RGD-BSA-PCL在肿瘤内信号强度明显强于131I-BSA-PCL。结论 131I标记的纳米脂质体131I-RGD-BSA-PCL对H460细胞裸鼠移植瘤具有明显的抑瘤作用，且131I-RGD-BSA-PCL能较长时间停留在肿瘤中。 |
| 英文摘要: |
| Objective To investigate the SPECT/CT imaging of non-small-cell lung carcinoma (NSCLC) mice models and the internal irradiation biological effects and therapeutic effectiveness of nanoliposome 131I-RGD-BSA-PCL. Methods RGD-BSA-PCL and BSA-PCL were constructed. The target binding and cellular uptake in H460 cell line were observed by fluorescence confocal microscopy in vitro. The nanoliposome with 131I were labeled using the Chloramine-T method. Apoptosis analyses was performed using flow cytometry. By construcing tumor xenografts, the biological distribution, change of tumor volume and the SPECT/CT imaging were discussed. Results Confocal microscopy revealed significant uptake of RGD-BSA-PCL or BSA-PCL in NCI-H460 cell after nanolipsome incubated for 1 and 8 h. The early apoptosis rates of Na131I, 131I-BSA-PCL and 131I-RGD-BSA-PCL were (33.3±12.5)%, (68.4±8.0)% and (70.5±12.2)%, respectively. The tumor uptake levels of 131I-BSA-PCL were higher than that of 131I-BSA-PCL (t=9.53, 5.03, P<0.01). There was a significant difference of tumor volume between the treatment group and the control group, and the tumor volume inhibition was most obvious in the 131I-RGD-BSA-PCL group on day 23 post-treatment (t=126.44, P<0.01). SPECT/CT tomography showed that 131I-RGD-BSA-PCL and 131I-BSA-PCL groups had obvious accumulation in the tumor on day 21 post-treatment, and intensity of radiation signal of 131I-RGD-BSA-PCL group was stronger than that of 131I-BSA-PCL group. Conclusions Radionuclide therapy using 131I-RGD-BSA-PCL, which showed excellent targeted cell killing and suppressed tumor growth, exhibited favorable intracellular retention of 131I. |
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