中华放射医学与防护杂志

原雅艺,张睿凤,董娟聪,张忠新,刘红艳,王超,段志凯,左雅慧.⁵⁶Fe¹⁷⁺、¹²C⁶⁺重离子束与⁶⁰Co γ射线对人淋巴细胞染色体畸变、细胞周期[J].中华放射医学与防护杂志,2016,36(8):572-576

⁵⁶Fe¹⁷⁺、¹²C⁶⁺重离子束与⁶⁰Co γ射线对人淋巴细胞染色体畸变、细胞周期

Effects of ⁵⁶Fe¹⁷⁺, ¹²C⁶⁺ heavy-ion and ⁶⁰Co γ-ray irradiation in chromosome aberrations, cell cycle and apoptosis of lymphocytes

投稿时间：2016-02-03

DOI：10.3760/cma.j.issn.0254-5098.2016.08.004

中文关键词: ⁵⁶Fe¹⁷⁺重离子 ¹²C⁶⁺重离子 ⁶⁰Co γ射线染色体畸变细胞凋亡

英文关键词:⁵⁶Fe¹⁷⁺ion beam ¹²C⁶⁺ion beam ⁶⁰Co γ-rays Chromosome aberrations Cell apoptosis

基金项目:

作者	单位	E-mail
原雅艺	030006 太原, 中国辐射防护研究院
张睿凤	030006 太原, 中国辐射防护研究院
董娟聪	030006 太原, 中国辐射防护研究院
张忠新	030006 太原, 中国辐射防护研究院
刘红艳	030006 太原, 中国辐射防护研究院
王超	030006 太原, 中国辐射防护研究院
段志凯	030006 太原, 中国辐射防护研究院
左雅慧	030006 太原, 中国辐射防护研究院	yahuiz@163.com

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中文摘要:

目的比较⁵⁶Fe¹⁷⁺、¹²C⁶⁺重离子束与⁶⁰Co γ射线对人淋巴细胞染色体畸变、周期、凋亡的影响。方法 ⁵⁶Fe¹⁷⁺、¹²C⁶⁺重离子束和⁶⁰Co γ射线分别照射人淋巴细胞系Peng-EBV，吸收剂量均为0、0.5和2.0 Gy，⁵⁶Fe¹⁷⁺重离子束剂量率为0.26～0.55 Gy/min，¹²C⁶⁺重离子束剂量率为0.30～0.50 Gy/min，γ射线照射剂量率为0.75 Gy/min。研究不同射线对染色体细胞畸变率、“双+环”畸变率的影响。利用流式细胞仪检测细胞周期和细胞凋亡。结果 ¹²C⁶⁺、⁵⁶Fe¹⁷⁺重离子与⁶⁰Co γ射线照射后细胞畸变率与0 Gy相比，差异有统计学意义（χ²=12.08～322.97，P<0.05）。“双+环”畸变率与0 Gy相比，差异有统计学意义（χ²=4.06～205.37，P<0.05）。其中，¹²C⁶⁺重离子束诱导的细胞畸变率和“双+环”畸变率最高，其次是⁵⁶Fe¹⁷⁺重离子束，而且二者均高于⁶⁰Co γ射线。⁵⁶Fe¹⁷⁺、¹²C⁶⁺离子束与⁶⁰Co γ射线均能诱导人淋巴细胞发生明显的G₂期阻滞，差异有统计学意义（t=-80.9～0.17，P<0.05），¹²C⁶⁺重离子、γ射线及⁵⁶Fe¹⁷⁺重离子诱导的G₂期阻滞程度依次降低。⁵⁶Fe¹⁷⁺、¹²C⁶⁺离子束与⁶⁰Co γ射线均能促进细胞凋亡，差异有统计学意义（t=-22.65～0.87，P<0.05），¹²C⁶⁺重离子、γ射线及⁵⁶Fe¹⁷⁺重离子诱导的早期凋亡率依次降低。结论 ⁵⁶Fe¹⁷⁺、¹²C⁶⁺离子束与⁶⁰Co γ射线均能诱导人淋巴细胞发生明显的染色体畸变、G₂期阻滞及细胞凋亡。¹²C⁶⁺离子束诱导的染色体畸变、周期阻滞和凋亡程度最高。⁵⁶Fe¹⁷⁺、¹²C⁶⁺离子束与γ射线的生物学效应与LET相关。

英文摘要:

Objective To compare the radiation effects of ⁵⁶Fe¹⁷⁺, ¹²C⁶⁺ion beams and ⁶⁰Co γ-rays on chromosome aberrations, cell cycle arrest and apoptosis induction in lymphocytes.Methods Lymphocytes Peng-EBV cells were exposed to ⁵⁶Fe¹⁷⁺, ¹²C⁶⁺ions and ⁶⁰Co γ-rays with different dose (0, 0.5, 2.0 Gy) at dose rates of 0.26-0.55, 0.30-0.50 and 0.75 Gy/min, respectively. Chromosome aberration and "d+r" distortion rate was assayed, and cell apoptosis and cell cycle were analyzed by flow cytometry. Results The percentage of cells with chromosome aberration increased significantly after exposure to¹²C⁶⁺, ⁵⁶Fe¹⁷⁺ion beams and ⁶⁰Co γ-rays(χ²=12.08-322.9, P<0.05), and the rate of "d+r" distortion was significantly increased as well(χ²=4.06-205.37, P<0.05). The chromosome aberration rate and "d+r" distortion rateinduced by ⁵⁶Fe¹⁷⁺and ¹²C⁶⁺ ion beams were higher than that of γ-rays, especially ¹²C⁶⁺ irradiation. The G\-2 phase arrest(t=-80.9-0.17,P<0.05)and cell apoptosis(t=-22.65-0.87,P<0.05)were significantly induced by each irradiation in an order of ¹²C⁶⁺, γ-rays and ⁵⁶Fe¹⁷⁺ ions from high to low. Conclusions Associated with the LET values, ¹²C⁶⁺, ⁵⁶Fe¹⁷⁺ ionbeams and ⁶⁰Co γ-rays could induce chromosome aberrations, G₂ phase arrest and apoptosis in human lymphocytes, where ¹²C⁶⁺ ion beam irradiation was much more effective.

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